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Example sentences for: schleicher

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2 μm, Schleicher and Schuell) in triplicate in denaturing solution (66 % formamide, 7.9 % formaldehyde, 26 mM MOPS buffer, pH 7.0, 1.3 mM EDTA, 0.066 M sodium acetate) using a slot blot apparatus.
Briefly 10 μg of total RNA from each sample was denatured at 65°C in RNA loading buffer, electrophoresed in a 1% agarose gel containing 2.2 M formaldehyde, then blotted onto a Nytran membrane (Schleicher & Schuell, Inc, Keene, N.H).
Equivalent amounts of protein were boiled in SDS sample buffer and separated by 10% SDS-PAGE and transferred onto nitrocellulose (Schleicher and Schuell).
All procedures were published previously [ 6 20 ] . In brief, protein samples of 25 μg in 2 × sodium dodecylsulfate loading buffer were fractionated by 10% sodium dodecylsulfate-polyacrylamide gel electrophoresis at 100 V. The proteins were transferred onto 0.45-μm pore nitrocellulose membrane (Schleicher & Schuell, Keene, NH) at 40 V for 45 to 60 minutes.
H441 cells were harvested and total RNA isolated as described previously [ 16 ] . Equal amounts of total RNA from each condition were separated on agarose gels, transferred to Nytran-plus membranes (Schleicher and Schuell, Keene, NH, USA), and subsequently hybridized with a radiolabeled human SP-A cDNA.