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Datasets scanned
The cDNA array has a long history of development [ 3 ] stemming from immunodiagnostic work in the 1980s; however, it has been most widely developed in recent years by Stanford University (California) researchers depositing cDNA tags onto glass slides, or chips, with precise robotic printers [ 4 ] . Labeled cDNA fragments are then hybridized to the tags on the chip, scanned, and differences in mRNA between samples identified and visualized using a variation of the red/green matrix originally introduced by Eisen and colleagues [ 5 ] . The light-generated oligonucleotide array, developed by Affymetrix, Inc. (Santa Clara, CA), involves synthesizing short 25-mer oligonucleotide probes directly onto a glass slide using photolithographic masks [ 6 7 ] . Sample processing includes the production of labeled cRNA, hybridization to a microarray, and quantification of the obtained signal after laser scanning.
Membranes were sequentially hybridized with radioactive probes for α2(I) procollagen and GAPDH and scanned with a PhosphorImager (Molecular Dynamics, Inc., Sunnyvale, CA) for mRNA quantitation.
Images were scanned with a Hewlett-Packard GeneArray scanner.
Probe arrays were scanned at 560 nm using a confocal laser scanning microscope (made for Affymetrix by Hewlett-Packard).