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Affinity-purified anti-α 2 β 1 integrin (MAB1988), anti-α v β 3 integrin (LM609), anti-MMP2 (MAB13405), and anti-VE-cadherin (MAB1989) monoclonal antibodies (mAbs) were purchased from Chemicon Intl; anti-Flt-1 (sc-316), anti-KDR (sc-6251), anti-Kinesin heavy chain (sc-13359), anti-Stanniocalcin (sc-14352), and anti-Grb2 were obtained from Santa Cruz Biotechnology Inc., and anti-β 1 integrin subunit mAbs (4B4), from Coulter Inc.
Immunohistochemical staining was done using rabbit polyclonal antibodies raised against synthetic peptides that correspond to regions in the mature forms of TGF-β 1 , TGF-β 2 and TGF-β 3 : anti-TGF-β 1 -LC and anti-TGF-β 1 -CC [ 19], anti-TGF-β 2 (sc-90; Santa Cruz Biotechnologies Inc, Santa Cruz, CA, USA), and anti-50-60-β 3 -LC [ 20], respectively.
MCMV strain Smith stock was purchased from American Type Tissue Collection (Rockville, MD) This virus stock had been propagated in SC-1 cells (mouse embryo fibroblast cell line).
5.4D for type I [ 23 ] , SC-71 for type IIa [ 24 ] , and BF-F3 for type IIb [ 24 ] . The antibody useful for the identification of type IIx fibers, BF-35 [ 24 ] , stains all fibers except pure IIx fibers.
Antibodies used were: actin (sc-8432), TGFβ 1 (sc-146), α V (sc-6617) and β 6 (sc-6632).