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Example sentences for: sc-
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Immunoblotting for the detection of Syk protein utilized a rabbit anti-Syk antibody generated against a different epitope (SC-1077, Santa Cruz Biotechnology).
Supernatants were recovered and incubated with protein-A agarose beads (Gibco BRL) coated with a rabbit anti-Syk antibody (SC-573, Santa Cruz Biotechnology, Inc.) for 30 min.
The following antibodies were used in a dilution of 1:1,000: TGFβ 1 (Santa Cruz [sc], sc-146), p-Tyr (sc-7020), β 6 -integrin (sc-6632), α V -integrin (sc-6617), p125 FAK(sc-557), TGFβ 1 -RI (sc-402), TGFβ 1 -RII (sc-400-G), ERK1/2-P (sc-7383), SMAD2/3 (sc-6033), SOS1/2 (sc-259), p130 cas(UBI-06-500), PCNA (sc-56), p21 WAF1(sc-6246), p27 KIP(sc-1641), c-fos (sc-7202), c-jun (sc-44), raf1 (sc-133), p21 Ras(sc-35) and phospho-threonine antibody (New England Biolabs, # 9381).
In blocking experiments, cells were preincubated with either a TGFβ 1 -RII-blocking antibody (R&D Systems # AF-241-NA, 15 μg/ml for 30 min), α V and β 6 -blocking antibodies (Santa Cruz, sc-6617 and sc-6632 respectively, 1:100 each for 30 min), or the MEK1 inhibitor PD98059 (New England Biolabs # 9900S, 7.5 ng/ml for 10 min) before stimulation with mature TGFβ 1 .
Antibodies used: rabbit Arp3 antibodies (gift from C. Egile using the same peptide as described [ 33 ] ) used at 1:500 dilution, mouse actin antibodies (Santa Cruz SC-8432) used at 1:10,000 dilution, rabbit VASP antibodies (gift from F. Southwick) used at 1:100, rabbit WAVE1 antibodies (gift from S. Eden as described [ 26 ] ) used at 1:100 and Alexa-dye labeled secondary antibodies, goat anti-rabbit and goat anti-mouse (Molecular Probes).
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