Words similar to sau
Example sentences for: sau
How can you use “sau” in a sentence? Here are some example sentences to help you improve your vocabulary:
The pYUB178 plasmid and the λ phage library of pYUB178::H37Rv cosmids were generously provided by Dr. William Jacobs, Albert Einstein University, New York, NY [ 20 ] . The cosmid library contains 30-50 kb fragments of M. tuberculosis genomic DNA generated by partial Sau 3A digestion cloned into Bcl I-digested pYUB178.
Sau 3AI was removed from the reaction by phenol-chloroform extraction.
These same cases were also screened for the exon 21 L858R mutation by a PCR–restriction fragment length polymorphism assay, based on a new Sau96I restriction site created by the L858R mutation (2,573T→G).
0 μg of genomic DNA was digested with 1 U Sau 3AI in 20 μl total volume for 2 min at 37°C and the reaction stopped by heating at 65°C for 10 min.
The Sau96I-digested fluorescently labeled PCR products were analyzed by capillary electrophoresis, and the following primers were used: EGFR -Ex21-FWD1, 5′- CCTCACAGCAGGGTCTTCTCTGT-3′, and EGFR -Ex21-REV1, 5′-Fam- TCAGGAAAATGCTGGCTGACCTA-3′.