Words similar to saponin
Example sentences for: saponin
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Fixed discs were then permeabilized in PBS containing 0.1% saponin and 1% goat serum and incubated overnight with an anti-E2F serum [ 12 ] at 1/250.
The cells were then grown in 12-well plates in triplicate wells, and measurement of 3H-hypoxanthine incorporation was carried out at 24 hr post-electroporation using standard procedures [ 49 ] , except that parasites were liberated with saponin, pelleted, and counts in the pellet measured following solubilization.
Though BDM does not inhibit nm-myosinII, in permeabilized yeast ( S. cerviseae ) BDM has been shown to decrease incorporation of exogenous actin into the cytoskeleton [ 20 ] . This process of actin incorporation requires proteins involved in actin filament formation and proteins that may localize a nucleation complex [ 20 21 ] . To determine if BDM has a similar effect on actin incorporation in mammalian cells, and if so an effect on actin nucleation, spreading Swiss3T3 cells were treated briefly with a media control or 15 mM BDM and then permeabilized with the detergent saponin in the presence of fluorescently labeled actin.
Cells were washed five times with ice-cold PBS containing 0.05% saponin and sonicated in 100 mM sodium acetate (pH 4.6).
On the other hand, when cells were fixed with PLP or LP and treated with 1% saponin in 3% BSA for permeabilization, most of the polypeptides were either not crosslinked by these relatively mild fixatives or crosslinks were reversed by the addition of SDS sample buffer (Figure 6B, lanes 4 and 5).
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