Words similar to saponin

Example sentences for: saponin

How can you use “saponin” in a sentence? Here are some example sentences to help you improve your vocabulary:

The cells were harvested, washed, fixed with paraformaldehyde, perme-abilized with saponin, and blocked with rodent serum.
Cytoplasmic IFNgR1 and IFNgR2 receptor subunits were detected by using a procedure described for intracellular cytokine staining [ 50 ] . Briefly, cells were fixed in ice-cold 4% formaldehyde for 5 min, washed 2 times, permeabilized in staining buffer containing 0.2% saponin for 60 min at 4°C, incubated with biotin-conjugated mAb's (specific or isotype-matched) for 30 min, washed 2 times, incubated with SA-PE and washed again (saponin was present in staining buffer at all times).
On the other hand, when cells were fixed with PLP or LP and treated with 1% saponin in 3% BSA for permeabilization, most of the polypeptides were either not crosslinked by these relatively mild fixatives or crosslinks were reversed by the addition of SDS sample buffer (Figure 6B, lanes 4 and 5).
The cells were then grown in 12-well plates in triplicate wells, and measurement of 3H-hypoxanthine incorporation was carried out at 24 hr post-electroporation using standard procedures [ 49 ] , except that parasites were liberated with saponin, pelleted, and counts in the pellet measured following solubilization.
Cells were harvested, fixed with formaldehyde, permeabilized with saponin, and intracellularly labeled for IFN-γ and IL-4, in addition to staining for surface CD69, CD4, and CD8 according to the manufacturer's recommendations (Fastimmune, BD Biosciences Pharmingen).