Words similar to saponin
Example sentences for: saponin
How can you use “saponin” in a sentence? Here are some example sentences to help you improve your vocabulary:
P. falciparum (3D7 or Dd2) was grown in human A-positive erythrocytes and infected cells were harvested at about 15% parasitemia and lysed by the saponin method essentially as described [ 14 15 ] . PfPP5 was partially purified using published procedures [ 18 ] . In brief, the parasites were resuspended in buffer A (50 mM Tris-Cl, pH 7.5, 10% glycerol, 1 mM DTT) plus 20 mM NaCl, 1% Triton X-100, leupeptin (1 μg/ml), and aprotinin (1 μg/ml) at an approximate protein concentration of 10 mg per ml.
Fixed discs were then permeabilized in PBS containing 0.1% saponin and 1% goat serum and incubated overnight with an anti-E2F serum [ 12 ] at 1/250.
Cytoplasmic IFNgR1 and IFNgR2 receptor subunits were detected by using a procedure described for intracellular cytokine staining [ 50 ] . Briefly, cells were fixed in ice-cold 4% formaldehyde for 5 min, washed 2 times, permeabilized in staining buffer containing 0.2% saponin for 60 min at 4°C, incubated with biotin-conjugated mAb's (specific or isotype-matched) for 30 min, washed 2 times, incubated with SA-PE and washed again (saponin was present in staining buffer at all times).
The cells were washed three times with ice-cold PBS containing 0.05% saponin.
The cells were then grown in 12-well plates in triplicate wells, and measurement of 3H-hypoxanthine incorporation was carried out at 24 hr post-electroporation using standard procedures [ 49 ] , except that parasites were liberated with saponin, pelleted, and counts in the pellet measured following solubilization.
Loading...