Words similar to saponin
Example sentences for: saponin
How can you use “saponin” in a sentence? Here are some example sentences to help you improve your vocabulary:
02 g/ml of saponin, 20 mM HEPES (pH 7.5), 138 mM KCl, 4 mM MgCl 2 , 3 mM ETGA, 1 mM DTT + protease inhibitors, 0.4 uM rhodamine actin, 1 mM ATP), and fixed in PBS + 3.7% formaldehyde for 10 min.
Other chemicals used were phorbol 12-myristate 13-acetate, dimethyl sulfoxide, ethanol, propidium iodide and saponin (all from Sigma).
Binding of β-glucuronidase was assayed as described previously [ 31 32 ] . Briefly, cells were permeabilized with 0.25% saponin in 50 mM Hepes (pH 7.0), 150 mM NaCl, 5 mM β-glycerophosphate, 0.5% human serum albumin, and 10 mM mannose-6-phosphate (M6P) for 30 minutes on ice.
The cells were then grown in 12-well plates in triplicate wells, and measurement of 3H-hypoxanthine incorporation was carried out at 24 hr post-electroporation using standard procedures [ 49 ] , except that parasites were liberated with saponin, pelleted, and counts in the pellet measured following solubilization.
Fixed discs were then permeabilized in PBS containing 0.1% saponin and 1% goat serum and incubated overnight with an anti-E2F serum [ 12 ] at 1/250.