Words similar to saci

Example sentences for: saci

How can you use “saci” in a sentence? Here are some example sentences to help you improve your vocabulary:

The construction of P51+6aa was carried out by removal of the 360 bp fragment from pKK-RT66+6aa by restriction digestion with SacI followed by re-ligation of the vector ends.
The PCR product was digested with SacI and XbaI and cloned into the SacI/XbaI sites of both pRS315 (pSW1264) and pRS316 (pSW1268) [ 66 ] . Plasmids with wild type SEC23 were constructed in the same manner using specific oligonucleotides (5' oligo sec23F5 with 5'-ATGAATATCTAGACCAGGGTGCC-3'; 3' oligo sec23-3 with 5'CCTTGGATCCGTAGTAAAGGCCACGCAG-3') and digestion of the PCR product with XbaI and BamHI to yield p SEC23/LEU2 (pSW1266) and p SEC23/URA3 (pSW1269).
The bpA sequence along with the 3' AscI site was excised from pbpAAsc by digesting it with SacI and KpnI, and inserted downstream of the MCS in pSAleotpA by digesting it with SacI and KpnI.
Next, pSAβgeo was digested with SacI to remove the SA and βgeo sequences, but leave behind the bpA, and self-ligated.
The probe used for nmt55/p54 nrbanalysis was a 499 bp SacI/BglII fragment representing the unique carboxyl terminus of nmt55/p54 nrb [ 13 ] . This probe was utilized to prevent cross-hybridization with other homologous RNA binding proteins.