Example sentences for: sacchi

How can you use “sacchi” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Total RNA was isolated from the liver tissue homogenate by using the method developed by Chomoczynski and Sacchi [ 23 ] . Briefly, chloroform was added and the aqueous phase containing the RNA was recovered.

  • Total RNA was extracted from the frozen mouse skin biopsies of 30 animals as described by Chomczynski and Sacchi (1987) [ 32 ] . The total RNA was digested by RNase free DNaseI and phenol extracted in order to remove genomic DNA contaminations.

  • Total cellular RNA was isolated from each gastrocnemius muscle by the method of Chomczynski and Sacchi [ 32 ] . RNA preparations were quantified by absorbance at 260 nm and RNA intactness and integrity assessed by ethidium bromide staining after separation by electrophoresis in a 6.6% formaldehyde-1% agarose gel.

  • Total RNA was prepared from synovial tissue by an acid phenol method according to the procedure described by Chomczynski and Sacchi [ 31 ] . RNA (20 μg) was digested with five units of DNase in 1× reverse transcription buffer (Gibco/Life Technologies, Grand Island, NY, USA) containing eight units of RNase inhibitor (Gibco/Life Technologies, Grand Island, NY, USA) for 30 minutes at 37°C.


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