Words similar to s-m

Example sentences for: s-m

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An RGS3-GST fusion protein was incubated with an 35S-Met-Gα-protein, and the amount of labeled Gα-protein was determined using glutathione-coupled beads to "pull down" the RGS3-GST/ 35S-Gα-protein complex that was then separated and quantified by SDS/PAGE (Fig.
Binding studies of recombinant RGS3-GST fusion protein to 35S-Met-Gα-proteins were performed as detailed [ 9 ] . Glutathione-coupled beads were used to select 35S-Gα proteins bound to RGS3-GST, and were then eluted for separation by SDS/PAGE [ 9 ] . The amount and molecular size of 35S-Gα protein were determined using a Phosphor imaging device (Molecular Dynamics).
RGS3 protein fused to glutathione-S-transferase (RSG3-GST fusion protein) was produced in the pGEX-5x-1 system of Pharmacia as detailed previously [ 9 ] . 35S-Met labeled Gα-proteins were prepared in vitro using a transcription/translation protocol [ 9 ] . cDNAs for Gqα [ 25 ] and Gzα [ 26 ] were obtained from M. I. Simon; for Gsα (ref.