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Example sentences for: ruppert

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pVL-GST-NFATp was created by subcloning a Nde I - EcoR I fragment containing the NFATp cDNA from pBS-KS +-NFATp(NdeI) into the Nde I and EcoR I sites of pVL1392-GST (gift of S. Ruppert and R. Tjian).
pBS-KS +-NFATp was generated by digesting pREP-NFATp with BssH II and Dra I, filling in the ends with Klenow, and ligating the resulting fragment into the Sma I site of pBS-KS +. pVL1392-HAX-NFATp was generated by subcloning a fragment excised from pBS-KS +-NFATp with Spe I and EcoR I into pVL1392-HAX (gift of S. Ruppert and R. Tjian) digested with Spe I and EcoR I. pBS-KS +-NFATp(NdeI), which has an Nde I site, containing the NFATp translational start site ATG, was generated in two steps.
The second way this model extends multiple logistic regression is that it accounts for the correlation within the repeated measures on individuals using individual specific random effects [ 21 ] . As discussed in Ruppert et al [ 22 ] we estimate the parameters in this model using the pseudo-likelihood method implemented by the SAS macro GLIMMIX [ 23 ] .