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Example sentences for: rubripes
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Three Runx genes were identified in the recently completed T. rubripes genome sequence by a TBLASTN search using the S. purpuratus Runt protein sequence as the query sequence at the Fugu BLAST Server of the UK Human Genome Mapping Project Resource Centre http://fugu.hgmp.mrc.ac.uk/blast/.
The sequences used in this study can be accessed through the NCBI website at http://www.ncbi.nih.gov, the Fugu rubripes website at http://genome.jgi-psf.org/fugu6/fugu6.home.html [ 47 ] and the FlyBase Consortium website at http://flybase.org/ [ 48 ] . The Fugu scaffold identities presented here refer to assembly v.3.
We were also able to identify the full-length genomic sequence of mosquito COP1 (Anopheles gambiae COP1 , AgCOP1 ) and partial genomic sequences of MmCOP1 and fugu COP1 (Fugu rubripes COP1 , FrCOP1 ). The genomic structures of the vertebrate COP1 genes appear to be generally conserved among themselves, but different from mosquito and plant COP1 genes (data not shown).
Also collected in the set for comparison were the previously-described Runx genes from D. melanogaster ( runt and lozenge [ 7 ] ), Caenorhabditis elegans ( run [ 8 ] ), sea urchin ( SpRunt [ 9 10 ] ), T. rubripes ( TrRunx2 [ 11 ] ), and mouse ( Runx1 , Runx2 , and Runx3 ; the three human RUNX genes, which have been extensively characterized previously, are highly similar to their mouse orthologues [ 12 13 ] and hence were not included in the collected set).
Whereas the genome of Fugu rubripes is one order of magnitude shorter than the length of the human genome, the total number of genes is estimated to be approximately the same [ 8 9 ] . Because of the low homology of amino- and carboxyl-terminal sequences, we were not able to determine the complete coding sequences of the eight pufferfish TMC proteins unequivocally; nevertheless, we obtained sufficient sequence information of the central parts of the proteins bearing the transmembrane domains to classify the eight pufferfish TMCs into the three subfamilies.
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