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Example sentences for: rubripes
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Also collected in the set for comparison were the previously-described Runx genes from D. melanogaster ( runt and lozenge [ 7 ] ), Caenorhabditis elegans ( run [ 8 ] ), sea urchin ( SpRunt [ 9 10 ] ), T. rubripes ( TrRunx2 [ 11 ] ), and mouse ( Runx1 , Runx2 , and Runx3 ; the three human RUNX genes, which have been extensively characterized previously, are highly similar to their mouse orthologues [ 12 13 ] and hence were not included in the collected set).
Analysis of the genome of Fugu rubripes (pufferfish) revealed that this duplication was even more ancient.
The sequences used in this study can be accessed through the NCBI website at http://www.ncbi.nih.gov, the Fugu rubripes website at http://genome.jgi-psf.org/fugu6/fugu6.home.html [ 47 ] and the FlyBase Consortium website at http://flybase.org/ [ 48 ] . The Fugu scaffold identities presented here refer to assembly v.3.
This subfamily assignment of individual TMC proteins was further substantiated by an analysis of the degree of conservation of intron positions within the Fugu rubripes TMC gene family (Figure 3B).
Whereas the genome of Fugu rubripes is one order of magnitude shorter than the length of the human genome, the total number of genes is estimated to be approximately the same [ 8 9 ] . Because of the low homology of amino- and carboxyl-terminal sequences, we were not able to determine the complete coding sequences of the eight pufferfish TMC proteins unequivocally; nevertheless, we obtained sufficient sequence information of the central parts of the proteins bearing the transmembrane domains to classify the eight pufferfish TMCs into the three subfamilies.
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