Example sentences for: rpci-
How can you use “rpci-” in a sentence? Here are some example sentences to help you improve your vocabulary:
STS marker D16S2986 was initially used to search the HTGS data and one clone RPCI-11_357N13 was retrieved with 100% identity.
To mark the regions of low-copy repeats in human 15q11-q13, we utilized a probe derived from the donor locus of a major component of the PWS/AS breakpoint duplication clusters, PAC clone pDJ-778A2 which contains a significant portion of the HERC2 gene in 15q13 [ 33 ] . In addition, we selected probes corresponding to sites distal to the PWS/AS domain flanking a large inverted-duplicated 15 (inv dup(15)) supernumerary marker chromosome [ 23 ] . Specifically, probe RPCI-11-88O16 (abbreviated RP11-88O16) was selected for FISH analysis because it lies distal to the inversion-duplication event breakpoint and is linked to STS marker D15S1010 [ 23 24 ] . Upon reaching the PTR inversion breakpoint interval with probe RP11-40J8, flanking BACs were chosen from the November 2002 human genome assembly to establish the extent of the interval and define the boundaries of the interval.
Gaps in the contigs were closed by a combination of DNA sequence alignments of BESs with HTGS data, clone walking using RNA probes from end clones to hybridize to the RPCI-11 gridded filters, and verification of clone positions by PCR for STSs.
Of 83 RPCI-11 clones in our contigs, 63 were present in the FPC database; 1 was not placed on a contig, 6 were placed on contigs of different chromosomes, and 56 in three chromosome 16 contigs, ctg16020, ctg16021 and ctg16024.
BACs were isolated from RPCI-11 male and RPCI-13 female BAC libraries as described [ 23, 24].
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