Words similar to rockford

Example sentences for: rockford

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Sympathetic neurons were dissociated from the SCG of perinatal (E21 to PN1) Holtzmann rats (Harlan Sprague-Dawley, Rockford, IL) according to previously described methods [ 59 ] . Cells were plated onto glass coverslips (for immunocytochemical and morphological studies) or 35 mm plastic culture dishes (for Western blot analyses) precoated with 100 μg/ml poly-D-lysine (Sigma, St. Louis, MO).
The concentration of protein in each synovial tissue and cell lysate was determined using a bicinchoninic acid assay (Pierce, Rockford, IL, USA), using BSA as the standard.
Cell extracts were spun at 14,000 rpm in a microcentrifuge and supernatants were incubated for 40-60 minutes with either anti-HA beads (Roche) or anti-v5 antibody (Invitrogen) and Protein A beads (Pierce; Rockford, USA) to form immunocomplexes.
The subunits were visualized using SuperSignal ECL reagent (Pierce, Rockford, IL).
Chemiluminescence was performed as described by the manufacturer (Pierce, Rockford, IL).