Example sentences for: rockford

How can you use “rockford” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cell extracts were spun at 14,000 rpm in a microcentrifuge and supernatants were incubated for 40-60 minutes with either anti-HA beads (Roche) or anti-v5 antibody (Invitrogen) and Protein A beads (Pierce; Rockford, USA) to form immunocomplexes.

  • Immunoblot (Western) detection by ECL was done with the SuperSignal Western blotting kit (Pierce Biotechnology, Rockford, IL) [ 10 ] .

  • Antibody-protein interactions were detected using enhanced chemiluminescence (ECL) (Pierce, Rockford, IL) and autoradiography.

  • For SDS/PAGE experiments on disulfide bonds, the oocytes were treated as above, except that the lysis buffer contained either 100 mM DTT or 20 mM Tris (2-Carboxyethyl) Phosphine Hydrochloride (TCEP) (Pierce, Rockford, IL).

  • Protein concentrations were determined using the BCA Protein Assay (Pierce, Rockford, IL).


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