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Example sentences for: rockford

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10% weight/volume protein homogenates were prepared from mouse brain as previously described [ 27 ] . Nuclear and cytoplasmic protein fractions were prepared with NE-PER Nuclear and Cytoplasmic Extraction Reagents using the manufacturer's instructions (Pierce Chemical Co., Rockford, IL).
Sympathetic neurons were dissociated from the SCG of perinatal (E21 to PN1) Holtzmann rats (Harlan Sprague-Dawley, Rockford, IL) according to previously described methods [ 59 ] . Cells were plated onto glass coverslips (for immunocytochemical and morphological studies) or 35 mm plastic culture dishes (for Western blot analyses) precoated with 100 μg/ml poly-D-lysine (Sigma, St. Louis, MO).
Biotinylation of Ad5.CMV-LacZ was performed by using sulfo-NHS-LC-biotin (Pierce Chemical, Rockford, IL), a biotin derivative containing an N -hydroxysuccinimidyl ester (NHS) that reacts with primary amino groups.
Immuncomplexes were detected by enhanced chemiluminescence (Pierce, Rockford, Illinois, United States).
The adherent cells in the well were washed three times with 1.0 ml of PBS, and the cells overlaid with 400 μl of lysis buffer (1% Triton X-100 in pH 7.5, 20 mM HEPES buffer with 0.1 mM EDTA), scraped, and collected into a glass vial for tocopherol analysis by HPLC, and for protein determination by the BCA protein assay (Pierce, Rockford, IL).