Words similar to roche
Example sentences for: roche
How can you use “roche” in a sentence? Here are some example sentences to help you improve your vocabulary:
The cell lysate was clarified at 13,000×g for 5 min and digested with 100 units micrococcal nuclease (Roche) for 45 min at 37°C in the presence of 10 mM CaCl 2 . Digestion was stopped by addition of EDTA to 15 mM, and then the lysate was clarified again by centrifugation at 13,000×g for 5 min.
-- Steve Roche
A 1:2000 dilution of anti-Elk-1 Ab (Santa Cruz Biotechnology) and a 1:5000 dilution of goat anti-rabbit secondary Ab (Roche Molecular Biochemicals) were used.
Labeled probe was synthesized using a High Prime random priming kit, according to the manufacturer's instructions (Roche Molecular Biochemicals, Indianapolis, IN), from 100 ng of genomic PCR product and 50 μCi [α- 32p]dCTP.
Proteins were separated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane (Roche) as described previously [ 66 ] . Blot membranes were blocked for 3 h at 37°C in PBS containing 5 % skim milk and probed with the respective antibodies (16 h at 4°C).