Example sentences for: roche

How can you use “roche” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cell lysate was clarified at 13,000×g for 5 min and digested with 100 units micrococcal nuclease (Roche) for 45 min at 37°C in the presence of 10 mM CaCl 2 . Digestion was stopped by addition of EDTA to 15 mM, and then the lysate was clarified again by centrifugation at 13,000×g for 5 min.

  • -- Steve Roche

  • A 1:2000 dilution of anti-Elk-1 Ab (Santa Cruz Biotechnology) and a 1:5000 dilution of goat anti-rabbit secondary Ab (Roche Molecular Biochemicals) were used.

  • Labeled probe was synthesized using a High Prime random priming kit, according to the manufacturer's instructions (Roche Molecular Biochemicals, Indianapolis, IN), from 100 ng of genomic PCR product and 50 μCi [α- 32p]dCTP.

  • Proteins were separated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane (Roche) as described previously [ 66 ] . Blot membranes were blocked for 3 h at 37°C in PBS containing 5 % skim milk and probed with the respective antibodies (16 h at 4°C).


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