Words similar to rkn
Example sentences for: rkn
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On the basis of both amino-acid sequence similarity (Table 3) and a Pfam [ 36 ] HMM search (e-value 4.3e -24), it is clear that the RKN glutamine synthetase is a GSI homolog, implying a prokaryotic origin.
Significantly, the solitary significant match of the Meloidogyne NodL sequences to a eukaryote is to a yeast serine-acetyltransferase, an enzyme clearly separated from the RKN by function as well as in our phylogeny (Figure 2).
Another RKN gene also postulated to have been acquired by HGT, and which encodes polyglutamate synthetase, was previously identified in M. artiellia [ 23 ] . Significantly, hybridization data showed that this particular gene is absent from both the M. javanica and G. rostochiensis genomes [ 23 ] . We speculate that acquisition of this gene by M. artiellia is a recent HGT event (event 'c', Figure 1), and thus it is truly absent from the Meloidogyne genomes from which our datasets were derived.
We wished to determine whether other RKN genes might have been acquired by horizontal gene transfer, particularly as such genes might potentially be related to parasitism.
The best match to a eukaryotic glutamine synthetase (GSII) is substantially lower (Table 3), strongly implicating the RKN gene as a robust candidate for an HGT event.