Words similar to rinsed

Example sentences for: rinsed

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Cells were rinsed with PBS and solubilized in a commercial mammalian extraction buffer (M-Per™; Pierce, Rockford, IL) supplemented with a protease inhibitor cocktail (Complete; Roche) followed by centrifugation at 12,000 × g for 5 min.
Sections were etched in 0.1 N HCl for 5 min., rinsed three times for five min each in TBS (25 mM Tris, 140 mM NaCl, 2.7 mM KCl, pH 8.0), placed in blocking buffer for 30 min (0.
After 1 h in an incubator, the cells were rinsed with fresh Mg 2+-free Locke's solution (NaCl, 154 mM; KCl, 5.6 mM; NaHCO 3 , 3.6 mM; CaCl 2 , 2.3 mM; glucose, 5.6 mM; HEPES, 5 mM; pH 7.4), and returned to the incubator for an additional 15 minutes prior to initiating the imaging experiments.
Subconfluent cultures of NIH3T3 cells on 3.5 cm plastic culture dishes, were rinsed with PBS(+), fixed with 4% paraformaldehyde in PBS(+) at room temperature or 37°C, or fixed and/or extracted by one of the procedures listed in Table 1. For some experiments, cells after fixation were treated twice with 0.5 mg/ml NaBH 4 in PBS for 10 min at room temperature.
BS-C-1 cells on 25 mm poly-L-lysine (PLL) coverslips were rinsed with media containing no antibiotics and placed in a 6 well plate.