Words similar to riboprobes

Example sentences for: riboprobes

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We used human insulin cDNA (320 bp) cloned in plasmid pCR4-TOPO (Invitrogen) as a template for in vitro transcription to produce riboprobes with a digoxigenin-RNA labeling kit (Ambion, Austin, Texas, United States), performed hybridization with IPC cluster sections as described [16], and used DIG Nucleic Acid Detection Kit (Roche, Indianapolis, Indiana, United States) for development according to the manufacturer's instructions.
Processing of tissue sections for hybridization and reactions with riboprobes were performed as described previously [ 39].
Slides, each of which contained lung tissue sections of both dexamethasone-immunosuppressed non-infected (NRL) and P. carinii -infected (PcIRL) rats, were probed with sense and anti-sense riboprobes of the ATPase 6 gene.
Radiolabeled riboprobes were synthesized from linearized plasmid DNA (1 μg) transcribed in vitro with 10 U of T3 RNA polymerase by using MAXIscript™ kit (Ambion) for 1 hour at 37°C in a total reaction volume of 20 μl.
EAE participated in the design, generated the riboprobes, performed the cryosections, the in situ hybrdizations, and immunohistochemistry, and drafted the manuscript.