Words similar to riboprobes

Example sentences for: riboprobes

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Radiolabeled riboprobes were synthesized from linearized plasmid DNA (1 μg) transcribed in vitro with 10 U of T3 RNA polymerase by using MAXIscript™ kit (Ambion) for 1 hour at 37°C in a total reaction volume of 20 μl.
Processing of tissue sections for hybridization and reactions with riboprobes were performed as described previously [ 39].
In situ hybridization with antisense riboprobes specific for human insulin mRNA labeled 25%–30% of stage 4 cells (see Figure 4H–4J), providing further evidence of insulin production.
Similarly, a 202 bp fragment was amplified from pCR21/4SP-b with primers SP-BF and SP-BR (see above) to produce SP-B riboprobes.
EAE participated in the design, generated the riboprobes, performed the cryosections, the in situ hybrdizations, and immunohistochemistry, and drafted the manuscript.