Words similar to riboprobes
Example sentences for: riboprobes
How can you use “riboprobes” in a sentence? Here are some example sentences to help you improve your vocabulary:
The RATPase6F-T7 adapter and SP-BF-T7 adapter PCR products were used as templates for in vitro transcription to produce antisense riboprobes, and the SP6 adapter-RATPase6R or SP6 adapter-SP-BR PCR PCR products were used to make sense probes.
Radiolabeled riboprobes were synthesized from linearized plasmid DNA (1 μg) transcribed in vitro with 10 U of T3 RNA polymerase by using MAXIscript™ kit (Ambion) for 1 hour at 37°C in a total reaction volume of 20 μl.
After in vitro transcription, 20 U of RNase-free DNase I was added to digest the DNA templates, and the labeled riboprobes were precipitated with ethanol and then resuspended in 100 μl of DEPC-treated water.
Similarly, a 202 bp fragment was amplified from pCR21/4SP-b with primers SP-BF and SP-BR (see above) to produce SP-B riboprobes.
The ATPase 6 clones were used as templates in PCRs with primer pairs RATPase6F (5'-ACCCCCATCTCACTAATTCC-3') and RATPase6R (5'-AGTACTAGGGTAGCTCCTCC-3') to generate a 145 bp fragment to make ATPase 6 riboprobes.