Words similar to riboprobes
Example sentences for: riboprobes
How can you use “riboprobes” in a sentence? Here are some example sentences to help you improve your vocabulary:
Radiolabeled riboprobes were synthesized from linearized plasmid DNA (1 μg) transcribed in vitro with 10 U of T3 RNA polymerase by using MAXIscript™ kit (Ambion) for 1 hour at 37°C in a total reaction volume of 20 μl.
After in vitro transcription, 20 U of RNase-free DNase I was added to digest the DNA templates, and the labeled riboprobes were precipitated with ethanol and then resuspended in 100 μl of DEPC-treated water.
in situhybridization of rat lung tissue using riboprobes
To verify over-expression of the ATPase 6 gene in rat lung tissue, in situ hybridization with digoxigenin-labeled riboprobes was performed.
For detection of hybridized DIG-labeled ATPase 6 riboprobes, hybridized sections of rat lung were incubated with mouse anti-DIG antibody followed by Texas Red-conjugated horse anti-mouse antibody and then Texas Red-conjugated rabbit anti-horse antibody.
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