Words similar to rgs
Example sentences for: rgs
How can you use “rgs” in a sentence? Here are some example sentences to help you improve your vocabulary:
To prepare hexa-histidine amino terminally-tagged RGS3T, we modified RGS3T cDNA [ 24 ] using PCR with primers containing restriction sites (the 5' primer incorporated a Bam HI site, whereas the 3' primer bore an Nde 1 site).
However, a calcium ionophore stimulated LH secretion in RGS3-expressing gonadotropes, confirming a specific site of action occurring at phospholipase Cβ or earlier in the signal transduction pathway.
cDNAs representing the GnRH receptor and RGS3 or RGS3T were co-transfected into COS-1 cells for GnRH-A treatment 72 h later; intracellular IP 3 concentrations were measured at 0, 10, 20, and 30 sec after addition of 10 -7M GnRH-A (Fig.
About 3 × 10 6pituitary cells infected, or not, 72 h previously with adeno-βgal or adeno-RGS3 were trypsinized, and cell membranes containing the GnRH receptors were prepared by placing the cells in a Dounce homogenizer (Kontes Co., Vineland, NJ) and disrupting them with 30 strokes of the pestle.
These results confirm our previous findings that RGS3 interacts with Gqα but also show that it has significant interaction with all other Gα-proteins studied.