Words similar to restriction-modification

Example sentences for: restriction-modification

How can you use “restriction-modification” in a sentence? Here are some example sentences to help you improve your vocabulary:

Game and his colleagues have developed a sensitive means of detecting chromosomal ds breakage using a circular chromosome [ 3 ] . Under most conditions of pulsed-field gel electrophoresis, a circular yeast chromosome and circular bacterial chromosomes will not enter the gel, very likely because they are trapped by the branches of the network of agarose [ 3 13 14 ] . One double-strand break transforms this circular form into a linear form, which can now move slowly in the gel [ 3 ] . We used this procedure to detect double-strand breakage of a circular bacterial chromosome occurring spontaneously or after loss of a restriction-modification gene complex [ 15 16 ] . We found increased chromosome breakage in recBC -null and recC1002 mutants of E. coli under both conditions [ 1 ] . Michel and her colleagues used pulsed-field gel electrophoresis to detect degraded chromosomal DNAs arising spontaneously in recBC mutants and arising during replication fork arrest [ 2 ] . RuvABC proteins, which catalyze migration and cleavage of Holliday junctions, are responsible for the occurrence of the degraded DNAs following replication fork arrests [ 17 ] .
These interpretations are based on a previous work with a yeast circular chromosome and on our analysis of E. coli chromosomal breakage after loss of restriction-modification genes [ 3 15 16 ] . In this work, we focus on the second DNA species - the huge linear forms in the area just below the well (Figure 1, marked by the bar).