Words similar to restimulation
Example sentences for: restimulation
How can you use “restimulation” in a sentence? Here are some example sentences to help you improve your vocabulary:
Blocking endogenous IL-4 using neutralizing antibodies has been shown [ 30] to result not only in the absence of T cells with a Th2-like phenotype, but also in the appearance of T cells producing IL-2 and IFN-γ after restimulation.
Blocking IFN-γ activity during in vitro priming with a neutralizing anti-IFN-γ antibody had little effect on the number of IFN-γ-secreting effector cells detected on subsequent restimulation.
After 72 hr activation, PBMC were washed and replaced in culture without stimulation for 36-40 hr prior to restimulation with 1 nM IL-2 [ 16 ] . To purify T lymphocytes, 72 hr OKT3-activated PBMC were depleted of CD56, and positively selected for CD2, using antibody-conjugated magnetic beads (anti-CD56 Polysciences Inc, anti-CD2 Dynal) according to manufacturer's instructions.
Subsequent to purification, T cells were replaced in culture for 36-40 hr prior to restimulation with 1 nM IL-2, and/or 10 μg/ml cycloheximide.
The cells were pre-activated for 72 hr with anti-CD3 to induce T cell IL-2 receptor expression, then washed and rested for 36-40 hr, prior to restimulation with IL-2 for 4 hr [ 16 ] .