Words similar to reprobed

Example sentences for: reprobed

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For protein load control, the membranes were reprobed with actin mouse mAb, clone C4 (Boehringer Mannheim Corp., Indianapolis, IN) at 0.25 μg/ml, and developed as above.
The membrane was stripped in 50% formamide, 0.1 × SSC, 0.1% SDS for 2 hr at 68°C, and reprobed with a cDNA for ACT1 (yeast actin gene).
The same Northern hybridization filters were reprobed with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) cDNA.
After initial blotting, membranes were reprobed for actin to ensure even loading.
Hybridization was visualized by autoradiography, and the filters were then stripped and reprobed with a [ 32P]labeled glyceraldehyde-3-phosphate dehydrogenase (GAPDH) cDNA probe to confirm equal loading.