Words similar to reprobed
Example sentences for: reprobed
How can you use “reprobed” in a sentence? Here are some example sentences to help you improve your vocabulary:
To ensure that all periplasmic contents were released during the spheroplast preparation, the blots were stripped of FLAG antibody and reprobed with antibody directed against MalE, a periplasmic binding protein.
Blots of cell lysates were stripped and reprobed using antibodies specific for α-tubulin (1:10,000, Sigma).
Hybridization was visualized by autoradiography, and the filters were then stripped and reprobed with a [ 32P]labeled glyceraldehyde-3-phosphate dehydrogenase (GAPDH) cDNA probe to confirm equal loading.
The membrane was stripped in 50% formamide, 0.1 × SSC, 0.1% SDS for 2 hr at 68°C, and reprobed with a cDNA for ACT1 (yeast actin gene).
Following immunodetection, all blots were stripped of primary antibody and reprobed with the precipitating antibody to verify that precipitation had occurred.