Example sentences for: reprobed

How can you use “reprobed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To ensure that all periplasmic contents were released during the spheroplast preparation, the blots were stripped of FLAG antibody and reprobed with antibody directed against MalE, a periplasmic binding protein.

  • Blots of cell lysates were stripped and reprobed using antibodies specific for α-tubulin (1:10,000, Sigma).

  • Hybridization was visualized by autoradiography, and the filters were then stripped and reprobed with a [ 32P]labeled glyceraldehyde-3-phosphate dehydrogenase (GAPDH) cDNA probe to confirm equal loading.

  • The membrane was stripped in 50% formamide, 0.1 × SSC, 0.1% SDS for 2 hr at 68°C, and reprobed with a cDNA for ACT1 (yeast actin gene).

  • Following immunodetection, all blots were stripped of primary antibody and reprobed with the precipitating antibody to verify that precipitation had occurred.


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