Example sentences for: reprobed

How can you use “reprobed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Blots of cell lysates were stripped and reprobed using antibodies specific for α-tubulin (1:10,000, Sigma).

  • The same Northern hybridization filters were reprobed with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) cDNA.

  • The membrane was stripped in 50% formamide, 0.1 × SSC, 0.1% SDS for 2 hr at 68°C, and reprobed with a cDNA for ACT1 (yeast actin gene).

  • Following immunodetection, all blots were stripped of primary antibody and reprobed with the precipitating antibody to verify that precipitation had occurred.

  • For protein load control, the membranes were reprobed with actin mouse mAb, clone C4 (Boehringer Mannheim Corp., Indianapolis, IN) at 0.25 μg/ml, and developed as above.


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