Words similar to reprobed
Example sentences for: reprobed
How can you use “reprobed” in a sentence? Here are some example sentences to help you improve your vocabulary:
Blots of cell lysates were stripped and reprobed using antibodies specific for α-tubulin (1:10,000, Sigma).
The same Northern hybridization filters were reprobed with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) cDNA.
The membrane was stripped in 50% formamide, 0.1 × SSC, 0.1% SDS for 2 hr at 68°C, and reprobed with a cDNA for ACT1 (yeast actin gene).
Following immunodetection, all blots were stripped of primary antibody and reprobed with the precipitating antibody to verify that precipitation had occurred.
For protein load control, the membranes were reprobed with actin mouse mAb, clone C4 (Boehringer Mannheim Corp., Indianapolis, IN) at 0.25 μg/ml, and developed as above.
Loading...