Words similar to replated
Example sentences for: replated
How can you use “replated” in a sentence? Here are some example sentences to help you improve your vocabulary:
Pituitary cells that had been re-trypsinized 72 h after infection and then replated (100,000 cells/well in a 24-well poly-L-lysine coated plate) for a 2 h preincubation were subsequently treated with a GnRH agonist (1 ml of 10 -7M D-Ala 6- des Gly 10- GnRH ethylamide; GnRH-A, Sigma, St. Louis, MO) or a calcium ionophore (A23187, Sigma) for 3 h as described by Conn et al.
24 h after transfection, cells were replated onto glass coverslips and grown for an additional 24 h before fixing in methanol at -20°C for 20 min.
After a further 24 h, the pituitary cells were recovered by trypinization, counted, and replated in balanced salt solution (400,000 - 500,000 cells/well in a 24-well plate) for a 2 h preincubation.
24 h after transfection, cells were replated into 6 cm plates.
CD73+ cells were replated in the absence of stromal feeders on tissue culture plates and expanded in alpha MEM medium with 20% FBS for 7–14 d.