Words similar to renilla
Example sentences for: renilla
How can you use “renilla” in a sentence? Here are some example sentences to help you improve your vocabulary:
To determine the luciferase activity, cells or tissues were lysed in Passive Lysis Buffer (Promega) and assayed for firefly (F-luc) and Renilla (R-Luc) luciferase according to the Promega Luciferase assay kit instructions in a Turner Designs (Sunnyvale, CA) TD-20/20 or a PharMingen Monolight 3010 luminometer.
Cells were simultaneously co-transfected by electroporation (Gene Pulser, Bio-Rad Inc.) with pGAL4-EcR, pVP16-mRXR, pRL-TK (Renilla luciferase) and pGAL4-EGFP.
In all experiments, luciferase activities were normalized with the renilla luciferase activity expressed from the pCMV-RL vector.
No change was seen in the level of the internal control Renilla luciferase with varying concentrations of GS-E (raw data not shown but effect accounted for in the calculation as defined above).
The DNA for transfection included 100 ng of pG4-TK-Luc reporter plasmid together with 50 ng of GAL4 and/or VP16 fusion vectors, and 10 ng of pCMV-RL vector (cytomegalovirus promoter fused to the renilla luciferase reporter gene [Promega]) as an internal control for transfection efficiency.