Words similar to religation
Example sentences for: religation
How can you use “religation” in a sentence? Here are some example sentences to help you improve your vocabulary:
Camptothecin (CPT) and its derivatives specifically target Topo I by binding noncovalently to these cleavable complexes, stabilizing them and interfering with DNA religation [ 11].
Even when E. coli was grown at lower temperatures, processing of the intein and religation of the exteins appeared 100% efficient.
Removal of negative supercoils from DNA by bacterial type IA topoisomerase involves the following steps: (1) binding of the enzyme to the junction of double-stranded and single-stranded DNA [ 8 ] ; (2) cleavage of a single-strand of DNA near the junction with cleavage sequence preference of a cytosine in the -4 position to form the covalent intermediate [ 9 10 ] ; (3) conformational change of the covalent enzyme-DNA complex to result in physical separation of the 5' phosphate covalently linked to the active tyrosine, and the 3' hydroxyl of the cleaved DNA; (4) passage of the complementary single strand through the break; (5) enzyme conformational change to bring the 5' phosphoryl end back into the proximity of the 3' hydroxyl group of the cleaved DNA; (6) religation of the phosphodiester bond.
Again the reversal of cleavage was complete for both Top67 and topoisomerase I within seconds after the addition of high salt and Mg 2+even when the reactions were carried out on ice (data not shown) so the lack of relaxation activity by Top67 is unlikely to be due to deficiency in religation.
A second enzyme conformational change positions the cleaved DNA ends for religation (step 4).
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