Words similar to religation
Example sentences for: religation
How can you use “religation” in a sentence? Here are some example sentences to help you improve your vocabulary:
However, it is possible that some enzyme preparations may contain bound Mg 2+and the low concentration of bound Mg 2+might have been sufficient for reversal of cleavage, as postulated previously to explain the data [ 14 ] . The results of this cleavage reversal experiment (Figure 5) indicated that the ZD domain was not required for efficient reversal of cleavage and Top67 could carry out religation of cleaved DNA.
Even when E. coli was grown at lower temperatures, processing of the intein and religation of the exteins appeared 100% efficient.
To test the religation capability of Top67, a 5'-end labeled oligonucleotide 61 base in length was first incubated with the enzyme in low ionic strength buffer to allow formation of the cleaved complex.
Removal of negative supercoils from DNA by bacterial type IA topoisomerase involves the following steps: (1) binding of the enzyme to the junction of double-stranded and single-stranded DNA [ 8 ] ; (2) cleavage of a single-strand of DNA near the junction with cleavage sequence preference of a cytosine in the -4 position to form the covalent intermediate [ 9 10 ] ; (3) conformational change of the covalent enzyme-DNA complex to result in physical separation of the 5' phosphate covalently linked to the active tyrosine, and the 3' hydroxyl of the cleaved DNA; (4) passage of the complementary single strand through the break; (5) enzyme conformational change to bring the 5' phosphoryl end back into the proximity of the 3' hydroxyl group of the cleaved DNA; (6) religation of the phosphodiester bond.
A second enzyme conformational change positions the cleaved DNA ends for religation (step 4).
Loading...