Example sentences for: religation

How can you use “religation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Camptothecin (CPT) and its derivatives specifically target Topo I by binding noncovalently to these cleavable complexes, stabilizing them and interfering with DNA religation [ 11].

  • Again the reversal of cleavage was complete for both Top67 and topoisomerase I within seconds after the addition of high salt and Mg 2+even when the reactions were carried out on ice (data not shown) so the lack of relaxation activity by Top67 is unlikely to be due to deficiency in religation.

  • Even when E. coli was grown at lower temperatures, processing of the intein and religation of the exteins appeared 100% efficient.

  • To test the religation capability of Top67, a 5'-end labeled oligonucleotide 61 base in length was first incubated with the enzyme in low ionic strength buffer to allow formation of the cleaved complex.

  • However, it is possible that some enzyme preparations may contain bound Mg 2+and the low concentration of bound Mg 2+might have been sufficient for reversal of cleavage, as postulated previously to explain the data [ 14 ] . The results of this cleavage reversal experiment (Figure 5) indicated that the ZD domain was not required for efficient reversal of cleavage and Top67 could carry out religation of cleaved DNA.


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