Words similar to refolded

Example sentences for: refolded

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Protein in positive elution fractions was refolded to native structure by serial dialysis against buffer D (20 mm HEPES, 0.1 M KCl, 0.2 mm EDTA, 0.5 mm dithiothreitol, 0.5 mm phenylmethylsulfonyl fluoride, 20% glycerol) containing stepwise decreasing concentrations of urea (6 M for 2 hours, 4 M for 2 hours, 2 M for 2 hours, no urea for 12 hours).
Interestingly, the specific inhibitory activity of wtPAI was 63 ± 15 U/ug just after purification, and was increased to 84 ± 21 U/ug after denatured by 6 M Gdn-HCl and refolded in 50 mM sodium acetate (pH 5.6) at 4°C.