Example sentences for: red-free

How can you use “red-free” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Three hours after transfection, medium was removed and hormones added in phenol red-free DMEM containing 5% charcoal stripped serum and antibiotics.

  • G418-resistant colonies from BRCA1 antisense infected BG-1 cells and control vector LXSN-infected BG-1 cells were pooled, pretreated for 5 days in phenol red-free, DMEM/F-12 medium (Gibco/Life Technologies) supplemented with 10% charcoal/dextran treated serum (Hyclone, Logan, UT, USA), then plated at 2.5 × 10 6cells per 100mm dish in triplicate for growth curve analysis in the absence or presence of estrogen (10 -8mol/l; 17β-Estradiol; 1,3,5 (10) - Estratriene 3, 17β-diol; Sigma, St Louis, MO, USA).

  • For soft agar assay, clones were plated into 10 60-mm dishes at 1 × 10 5cells/dish containing 0.3% bactopeptone agar with or without added estrogen (10 -8mol/l) in phenol red-free medium with 10% stripped serum in order to test for anchorage independent growth.

  • Selected BG-1 clones were tested for anchorage independent growth in 0.3% bacto-peptone agar with a 0.6% bacto-peptone agar base plus or minus added estrogen (10 -8mol/l) in phenol red-free medium with 10% stripped serum.

  • Growth curve analysis of Infected populations and were pretreated for 5 days in phenol red-free, Dulbecco's modified eagle medium (DMEM)/F-12 medium (Gibco/Life Technologies) supplemented with 10% charcoal/dextran treated serum (Hyclone, Logan, UT, USA), then plated at 2.5 × 10 6cells per 100mm dish in triplicate in the absence or presence of estrogen (10 -8mol/l; 17β-Estradiol; 1,3,5 (10) - Estratriene 3,17β-diol; Sigma, St Louis, MO, USA).


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