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Example sentences for: recovered

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The protein fractions with enzymatic activity that eluted from the gradient of a hydroxylapatite column were chromatographed on a mono Q column and recovered as single peaks.
This is consistent with previous reports of Tn 5 preferentially inserting into particular locations in the target sequence [ 17 18 ] . Since these "hotspots" could become a limiting factor in the number of unique insertions recovered within a target sequence, the second reading frame through the Tn 5 MEs was used for the ECFP transposon.
The aqueous phase was recovered following centrifugation at 14 000 rpm for 20 min at 4°C and added to a tube containing 500 μl of chloroform, vortexed briefly and centrifuged at 10 000 rpm for 10 min at 4°C.
The complexes were recovered with Protein A-Sepharose (Sigma) and washed two times with ice cold NP40 wash buffer (1% NP40, PBS, 1 mM sodium orthovanadate) and analyzed by Western blotting using either anti-Rlk serum or anti-Fyn serum (1:1000).
The numbers of L3 larvae recovered declined significantly in only one of the 4 sentinel sites (Figure 1, Table 2).