Example sentences for: recircularization

How can you use “recircularization” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To reduce the background caused by recircularization of ARS -containing vectors, we developed a different gene capture strategy (Figure 2).

  • The use of the system allowed us to include a yeast origin of replication into the TAR vector and to select TAR cloning events against vector recircularization.

  • A plasmid was constructed that carried 825 bp from the pseudokinase domain of ifkA and 731 bp from the kinase domain, placed at either end of a 1.4 kb blasticidin resistance cassette [ 48 ] . A 3.95 kb EcoR I/ Hind III fragment, containing the ifkA sequences and the blasticidin gene, was released from the plasmid and it's ends were made blunt using the klenow fragment and dephosphorylated to prevent recircularization and reduce nonspecific integration of the fragment.

  • Negative genetic selection eliminates the background due to vector recircularization caused by end-joining during yeast transformation.


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