Example sentences for: reassociated

How can you use “reassociated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The reassociated DNA was used as a template for the PCR direct labeling.

  • A fraction of each mixture was reassociated by denaturing at 95°C for 5 min and annealing at 68°C for 20 hr.

  • With indirect labeling, aminoallyl-dUTP is incorporated into control and tester DNA with PCR of 3 cycles after the reassociated DNA templates are amplified.

  • Based on the current working protocol, 10 μg of total RNA from control and tester samples was used to generate 160 μl of reassociated DNA.

  • To generate the probe of HL60 DNA, a PCR reaction was set up with 0.5 μl of the reassociated DNA, 5 μl of 10x Clontech PCR buffer, 1 μl of dNTPS (10 mM dATP, dTTP, and dGTP each, 6 mM dCTP), 4 μl of FluoroLink Cy3-dCTP (Amersham Pharmacia), 4.5 μl of 10 μM CT primer, 1 μl of Clontech cDNA polymerase and 34 μl of ddH 2 O. In the PCR reaction for the probe of HL60/TLK286 DNA, Cy5-dCTP and TT primer were used instead of Cy3-dCTP and CT primer.


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