Words similar to reassociated
Example sentences for: reassociated
How can you use “reassociated” in a sentence? Here are some example sentences to help you improve your vocabulary:
The reassociated DNA was used as a template for the PCR direct labeling.
A fraction of each mixture was reassociated by denaturing at 95°C for 5 min and annealing at 68°C for 20 hr.
With indirect labeling, aminoallyl-dUTP is incorporated into control and tester DNA with PCR of 3 cycles after the reassociated DNA templates are amplified.
Based on the current working protocol, 10 μg of total RNA from control and tester samples was used to generate 160 μl of reassociated DNA.
To generate the probe of HL60 DNA, a PCR reaction was set up with 0.5 μl of the reassociated DNA, 5 μl of 10x Clontech PCR buffer, 1 μl of dNTPS (10 mM dATP, dTTP, and dGTP each, 6 mM dCTP), 4 μl of FluoroLink Cy3-dCTP (Amersham Pharmacia), 4.5 μl of 10 μM CT primer, 1 μl of Clontech cDNA polymerase and 34 μl of ddH 2 O. In the PCR reaction for the probe of HL60/TLK286 DNA, Cy5-dCTP and TT primer were used instead of Cy3-dCTP and CT primer.
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