Words similar to reassociated

Example sentences for: reassociated

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A PCR reaction was set up with 1 μl of the reassociated DNA, 5 μl of 10x Clontech PCR buffer, 1 μl of 10 mM dNTPS, 2 μl of 10 μM CT primer or TT primer, 1 μl of Clontech cDNA polymerase and 40 μl of ddH 2 O. The reaction cycling conditions were 72°C for 5 min (for filling in the adapter ends), 94°C for 1 min, then 15 or 20 cycles of 94°C for 30 s, 66°C for 30 s, 72°C for 90 s, then 72°C for a final extension.
A fraction of each mixture was reassociated by denaturing at 95°C for 5 min and annealing at 68°C for 20 hr.
The reassociated DNA is amplified by using PCR with the CT primer complementary to the CT adapter or the TT primer complementary to the TT adapter.
Based on the current working protocol, 10 μg of total RNA from control and tester samples was used to generate 160 μl of reassociated DNA.
The adapter-linked control and tester DNA are reassociated through mixing at a ratio of 1:1, denaturing and annealing.