Words similar to reassociated

Example sentences for: reassociated

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A PCR reaction was set up with 1 μl of the reassociated DNA, 5 μl of 10x Clontech PCR buffer, 1 μl of 10 mM dNTPS, 2 μl of 10 μM CT primer or TT primer, 1 μl of Clontech cDNA polymerase and 40 μl of ddH 2 O. The reaction cycling conditions were 72°C for 5 min (for filling in the adapter ends), 94°C for 1 min, then 15 or 20 cycles of 94°C for 30 s, 66°C for 30 s, 72°C for 90 s, then 72°C for a final extension.
2 μl of the reassociated DNA was needed to make probe for one slide hybridization.
The reassociated DNA was used as a template for the PCR direct labeling.
A fraction of each mixture was reassociated by denaturing at 95°C for 5 min and annealing at 68°C for 20 hr.
The adapter-linked control and tester DNA are reassociated through mixing at a ratio of 1:1, denaturing and annealing.