Words similar to read-through
Example sentences for: read-through
How can you use “read-through” in a sentence? Here are some example sentences to help you improve your vocabulary:
A shorter variant contained the intact 3' end of exon 7 and 60 nt of read-through transcription into the ETn element spliced to exon 8 (7+60ETn,8).
The longer variant contained the intact 3' end of exon 7 and 60 nt of read-through into the ETn element spliced to a 37 nt mini-exon of ETn sequence, then spliced to exon 8 (7+60ETn,+37ETn,8).
In the T Wis / T Wis embryo, this primer set detected a mutant transcript representing read-through transcription from exon 7 into the immediately 3' ETn insertion (6,7+Etn; Figure 1B, Table 2, Figure 3C).
Given that the BGH037 primer is contained within the 60 nt of ETn read-through, this species is not necessarily distinct from the 6,7+ETn transcript amplified by F1-BGH037, as both could be amplified from the same hypothetical RNA 6,7+60ETn,8.
They showed that the splice site at the 3' end of exon 7 was mutated from TAG GTATGT to TAG GTGTTG (where underlined sequence is the 3' end of exon 7 and ETn insertion sequence is in bold) and predicted that this site would be nonfunctional, thereby abolishing splicing of exon 7 to exon 8. They proposed that the T Wis allele would produce a transcript comprised of exons 1 through 7 followed by read-through transcription of the ETn element, resulting in a modified C-terminal end, or alternatively, that upstream splice donor sites would be used, shortening the transcript and protein product.