Example sentences for: re-ligated

How can you use “re-ligated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • pEGFP-N280 was digested with EcoRI and BamHI, filled in with Klenow DNA polymerase on both ends, and re-ligated to generate pEGFP-N200.

  • The 12 clones encoding unique α s - GFP fusion proteins were digested with Srf I and re-ligated to create full-length fusion proteins (figure 2B).

  • Clones representing unique fluorescent fusion proteins were digested with Srf I to remove the Kan r selection cassette and re-ligated to generate full-length GluR1-EGFP/ECFP fusions.

  • This frame-shift mutation was re-ligated into an otherwise full-length α 1S , subcloned into the mammalian expression vector pSG5, and transfected into dysgenic (α 1S null) myotubes.


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