Words similar to rbx
Example sentences for: rbx
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Studies in budding yeast identified SCF Cdc4p, an E3 ubiquitin ligase complex that mediates the ubiquitylation of the CDK inhibitor Sic1p [ 3 4 ] . SCF Cdc4pconsists of at least four proteins: the cullin Cdc53p, the RING domain protein Hrt1p/Rbx1p/Roc1p, the adapter protein Skp1p, and Cdc4p (reviewed in [ 1 ] ). Cdc4p contains two sequence motifs, which are conserved in a wide variety of so-called F-box proteins: C-terminal WD-repeats that are involved in binding the substrate Sic1p in a phosphorylation-dependent manner, and a central F-box [ 5 ] that interacts with Skp1p [ 6 7 ] . Cdc53p in turn binds to Skp1p and Hrt1p/Rbx1p/Roc1p, which bridges Cdc53p with the ubiquitin-conjugating enzyme Cdc34p/Ubc3p [ 6 8 9 10 11 12 ] . In vitro reconstitution demonstrated that SCF Cdc4p, Cdc34p, E1, ubiquitin, and ATP are sufficient to mediate Sic1p polyubiquitylation [ 6 7 ] .
Based on the composition of known SCF complexes, we identified in the S. pombe genome database psh1 (pombe skp1 homologue) and pip1 (pop interacting protein 1), two genes encoding proteins with strong similarity to human SKP1 and HRT1/RBX1/ROC1, respectively (data not shown).
In addition to the key roles in E3 ligase activity, RING finger domains have also been shown to be important for regulating subcellular localization of E3s and/or their substrates in a number of cases [ 53 54 55 56 ] . Studies have shown the MDM2 RING finger domain is required to promote nuclear export of p53 and this activity of MDM2 RING finger seems to be coupled with its ability to promote ubiquitination of p53 [ 53 54 55 ] . Rbx1/ROC1, the small RING finger subunit of the SCF E3 complexes, promotes the nuclear accumulation of Cul1, another subunit of the SCF complexes [ 56 ] . These studies have suggested a correlation between the ubiquitination activities of RING fingers and their roles in regulating subcellular localization of the E3 ligases which they are part of.
SCF complexes share homologues of the core components cullin 1 (CUL1), SKP1, and the RING domain protein HRT1/RBX1/ROC1, which associate with different F-box proteins and ubiquitin-conjugating enzymes [ 1].
SCF complexes are E3s composed of the Cullin family member Cul1 (Cdc53 in S. cerevisiae ), as well as, Skp1, Roc1 (also called Rbx1 or Hrt1) and one of the numerous F-box proteins that act as a receptor for the substrate (for review see [ 2 ] ).
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