Words similar to rauwolscine

Example sentences for: rauwolscine

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The extent of down-regulation was determined using a single concentration of [ 3H]rauwolscine (0.
113 nM) and similar to the previously reported K d of 0.056 nM for the wild-type alpha-2 receptor for [ 3H]rauwolscine in NaPO 4 buffer [ 23 ] . Inhibition experiments with [ 3H]rauwolscine indicated that the affinity of norepinephrine for the mutated receptors was approximately two-fold higher (lower K d value) than for wild-type receptor (Figure 3).
Wild-type and mutated receptors in the pRc/CMW vector were transfected into CHO-K1 cells using calcium phosphate precipitation technique as described previously [ 21 ] . Stable transfectants were selected with 500 μg/ml G-418 (Geneticin; Life Technologies) and screened by intact cell binding using [ 3H]rauwolscine as previously described [ 21 ] .
For saturation experiments, duplicate tubes were prepared containing 970 μl of tissue and 20 μl of [ 3H]rauwolscine at six concentrations, with final concentrations from approximately 6 pM to 0.6 nM.
For competition experiments, duplicate tubes were prepared containing 970 μl of tissue, 10 μl of norepinephrine at various concentrations, and 20 μl of [ 3H]rauwolscine (81.