Words similar to ratiometric
Example sentences for: ratiometric
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The pH-dependency of fluorescence of both oxidized OxyBURST Green H 2 HFF BSA and 5-(and -6)-carboxyfluorescein were thus examined by the dual excitation ratiometric measurement in vitro , as shown in Figure 8. While 5-(and -6)-carboxyfluorescein exhibits strong pH-dependency, the experiment clearly demonstrates that fluorescence of the oxidized OxyBURST Green H 2 HFF BSA is not affected by variation of the pH within a range between 4.5 to 7.5.
Imaging analysis to calculate the ratio of green fluorescence in lysosomes under excitation by 485 and 440 nm (" [F 535 (λ 485 )/F 535 (λ 440 )]") was carried out as previously described [ 3 ] . The ratiometric data were converted to pH using a calibration curve from the lysosomes of ionophore-clamped cells.
Averaged lysosomal pH was measured in control cells or cells treated with PMA, using fluorescein-conjugated dextran in dual-excitation ratiometric mode [ 8 ] . The validation of lysosomal pH measurements was performed by the calibration curve using ionophore-clamped cells as shown in Figure 3. The ratio of emission fluorescence under excitation at 485 nm versus 440 nm (i.e.
1 cells was measured by a dual excitation ratiometric measurement according to established procedures using the fluorescein-conjugated dextran [ 3 8 ] . Namely, cells were incubated with 2-5 mg/mL fluorescein-conjugated dextran for approximately 18 hours in culture medium.
Most interestingly, a heterogeneous distribution of lysosomes with elevated pH was observed by ratiometric images as shown in Figure 4. Here, lysosomal pH in untreated cells is very homogeneous, and estimated to be 4.3-4.
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