Words similar to rab
Example sentences for: rab
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It is well established that mutations affecting the guanine nucleotide state of Ras are associated with neoplastic transformation of mammalian cells [ 35 72 73 ] . However, the potential role of Rab mutations in disease is just beginning to be explored.
Aside from its well-characterized role in protein folding [ 66 ] , Hsp70 is known to associate with the GR in the cytoplasm, where it promotes formation of a heterocomplex between the inactive GR and Hsp90 [ 67 ] . In addition, Hsp70 becomes concentrated in the nucleus when cells are exposed to environmental stress [ 68 ] . Of particular note, the nuclear accumulation of the Hsp70, Ssa4p, in response to nutrient deprivation in yeast appears to occur by a novel mechanism that involves its direct interaction with Mmd5p, a member of the importin-β family [ 69 ] . A role for Rab24 in nuclear trafficking would be unanticipated, since the only GTPase known to function in nuclear import/export (Ran) is structurally distinct from the Rab subgroup of the Ras superfamily [ 70 71 ] . Nevertheless, we can speculate that if Rab24 participates in a stress-induced pathway for nuclear import of Hsp70 in mammalian cells, the constitutive overexpression of Rab24(D123I) might stimulate aberrant accumulation of Hsp70/importin-β aggregates at the nuclear pore complex.
Confirmatory studies with other potential dominant-negative mutants, such as those bearing substitutions at the position cognate to Ras S17, were not possible with Rab24, because such mutants remained in the GTP state.
Here we have shown that single amino acid substitutions ( e.g. , D123I and T120A) in one of the conserved guanine nucleotide binding domains of Rab24 can cause the protein to accumulate in a massive array of nuclear inclusions in mammalian cells.
Figure 4shows a series of representative photographs of transfected 3T3 cells that were co-stained with antibody against the Rab24 myc epitope, combined with DAPI to visualize the chromatin (Fig.
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