Example sentences for: rab

How can you use “rab” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • , IL) as described previously [ 50 81 ] . To determine the detergent solubility of the Rab24(D123I) mutant, transfected cells from a 10 cm culture dish were homogenized in 0.2 ml of 100 mM Tris-HCl, pH 7.4, containing 0.5% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, and 2 mM EDTA.

  • Since neither Rab24(S22N) nor Rab24(S67Q) showed evidence of being locked in a GDP-bound state, it is unlikely that either would function as a dominant-negative.

  • To detect the expressed myc-tagged Rab proteins, cells were incubated for 60 min with either the 9E10 mouse monoclonal antibody against the myc epitope or a rabbit polyclonal antibody against the same epitope (Upstate Biotechnology, Lake Placid, NY).

  • Western blot analyses of cytosol and particulate fractions obtained from the transfected 293 cells indicated that mycRab24(wt) was present in both fractions, consistent with previous results with Rab24 and other wild-type Rab GTPases [ 25 39 ] . In contrast, mycRab24(D123I) was found exclusively in the particulate fraction (Fig.

  • Several follow-up studies supported the notion that the punctate structures containing the Rab24 mutants were inclusion bodies rather than membrane-bound organelles.


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