Example sentences for: rab

How can you use “rab” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The nuclear inclusions containing Rab24(D123I) were not cell-type specific.

  • Solubilization of Rab24(D123I) from inclusions and assay for ubiquitination

  • Oligonucleotide primers for the PCR reaction were based on the published sequence of murine Rab24 [ 24 ] . Kpn1 and BamH1 restriction sites were added to the 5' and 3' primers respectively, to facilitate cloning of the PCR product in frame with a 5' sequence cassette encoding a myc epitope (EQKLISEEDL), previously introduced into the pCMV5 expression vector [ 78 ] . The Rab24 cDNA was altered by overlap-extension PCR [ 79 ] , using appropriate mutator oligonucleotides and Pfu DNA polymerase (Stratagene Inc., La Jolla, CA), so as to introduce amino acid substitutions at D123 (D123I), T120 (T120A), S22 (S22N), S67 (S67Q), and a deletion of residues 127 through 130 (ΔEDRR).

  • Ultimately, the accumulation of nuclear inclusions in cells expressing Rab24(D123I) causes a catastrophic disruption of the nuclear architecture, although this does not appear to be accompanied by classic signs of apoptotic cell death such as DNA fragmentation or caspase activation.

  • We also verified that the unique behavior of the Rab24 mutant was not related specifically to the substitution at the aspartate position in the N(T)KxD motif.


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