Words similar to rab

Example sentences for: rab

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Samples of the eluted protein were subjected to SDS-PAGE and immunoblot analysis as described previously, using a polyclonal antibody against Rab24 [ 25 ] , or monoclonal antibodies against the His 6 -tag (Sigma) or ubiquitin (Zymed Laboratories).
To generate Rab24 fusions with Enhanced Green Fluorescent Protein (EGFP), cDNAs encoding full-length Rab24(wt) or Rab24(D123I), or the Rab24 C-terminal hypervariable domain (residues 166-203) were generated with flanking KpnI and BamHI sites and subcloned into pEGFP-C1 (Clontech Inc., Palo Alto, CA) so that the Rab inserts were in-frame with a 5' EGFP coding sequence.
Upon addition of 1 μM dexamethasone (Dex) the receptor is translocated into the nucleus within 10 min [ 48 ] . When GR-GFP was co-expressed with the wild-type Rab24 in NIH3T3 cells, we observed normal ligand-dependent translocation of GR-GFP into the nucleus when Dex was applied (Fig.
Rab24 is known to be expressed endogenously in many cell types, including the cell lines used in these studies [ 24 25 ] , and we have estimated that both mycRab24wt and D123I are transiently expressed 5-10 fold over the level of endogenous Rab24 (data not shown).
Other pCMV5 expression vectors encoding Rab1B(N121I) [ 28 ] , Rab6(N126I) and Rab8(N121I) [ 32 ] have been described in the indicated references.