Words similar to quiesced

Example sentences for: quiesced

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Work in our laboratory has shown that recognition of ERK by anti-phospho-ERK antibodies correlates with an increase in ERK activity [ 21 30 31 ] . Interestingly, the anti-phospho-ERK immunoblots revealed that ERKs were constitutively activated in untreated, quiesced MDCK cells and activation was not enhanced further by ATP or IONO (Fig.
MDCK cells grown on MatTek plates were quiesced overnight in serum-free medium, washed with HHBSS containing 1 mM probenecid and incubated with 5 μM Fura2-AM (Calbiochem) in HHBSS, 1 mM probenecid, and 1% DMSO for 45 min at 37°C.
EYFP-cPLA 2 /ECFP-cPLA 2 S505A- or pECFP-cPLA 2 S727A-transfected MDCK cells grown on MatTek plates were quiesced overnight in serum-free medium, washed with and incubated in Hank's balanced salt solution (HBSS) additionally buffered with 25 mM HEPES pH 7.4 (HHBSS).
Stable EGFP-cPLA 2 transfectants were grown on 100 mm dishes at 5 × 10 3cells/cm 2in growth medium for one day, then quiesced in serum-free medium overnight.
To study the role of the MEK1/ERK pathway in cPLA 2 activation, quiesced MDCK cells were treated with the MEK1 inhibitor U0126, and the effect on AA release, ERK activation, and cPLA 2 gel shift determined (Fig.