Words similar to quiesced

Example sentences for: quiesced

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Work in our laboratory has shown that recognition of ERK by anti-phospho-ERK antibodies correlates with an increase in ERK activity [ 21 30 31 ] . Interestingly, the anti-phospho-ERK immunoblots revealed that ERKs were constitutively activated in untreated, quiesced MDCK cells and activation was not enhanced further by ATP or IONO (Fig.
Stable EGFP-cPLA 2 transfectants were grown on 100 mm dishes at 5 × 10 3cells/cm 2in growth medium for one day, then quiesced in serum-free medium overnight.
MDCK cells grown on MatTek plates were quiesced overnight in serum-free medium, washed with HHBSS containing 1 mM probenecid and incubated with 5 μM Fura2-AM (Calbiochem) in HHBSS, 1 mM probenecid, and 1% DMSO for 45 min at 37°C.
EYFP-cPLA 2 /ECFP-cPLA 2 S505A- or pECFP-cPLA 2 S727A-transfected MDCK cells grown on MatTek plates were quiesced overnight in serum-free medium, washed with and incubated in Hank's balanced salt solution (HBSS) additionally buffered with 25 mM HEPES pH 7.4 (HHBSS).
To study the role of the MEK1/ERK pathway in cPLA 2 activation, quiesced MDCK cells were treated with the MEK1 inhibitor U0126, and the effect on AA release, ERK activation, and cPLA 2 gel shift determined (Fig.