Example sentences for: q-pna
How can you use “q-pna” in a sentence? Here are some example sentences to help you improve your vocabulary:
The reaction was cooled to 4°C, and 20 μl ERCA reaction mix was added (typically 16 units BST polymerase (New England Biolabs, Beverly, MA), 6 mM dNTPs, 0.5 μM P1, 0.5 μM P2, 4 μM Q-PNA, 7.5 μM TMAO in 1x ThermoPol Buffer II, all concentrations final).
The C-terminal dabcyl was positioned so that it was proximal to the 5'-fluorescent moiety on the DNA reporter, quenching fluorescence when the reporter and Q-PNA-13 were annealed, as shown in Figure 2. After P1 primer is incorporated into ERCA product, Q-PNA is displaced, producing a fluorescent signal.
The Q-PNA detection system [ 9 ] was used as a fluorescent reporter during ERCA.
Q-PNA
The genotyping signals appeared in as little as 10 minutes using the Q-PNA detection system.
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