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Example sentences for: pyub

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Bacteria from ~4,000 colonies (representing ~20 genome equivalents) were recovered and pooled to generate the M. smegmatis (pYUB178::H37Rv) library.
In the λ phage library, ~225 cosmids represent one genome-equivalent of M. tuberculosis [ 20 ] . An E. coli (pYUB178::H37Rv) library was created by infecting E. coli XL1-Blue with the λ phage library.
The pooled pYUB178::H37Rv cosmid DNAs were electroporated into M. smegmatis LR222, and kanamycin-resistant colonies were isolated.
The pYUB178 plasmid and the λ phage library of pYUB178::H37Rv cosmids were generously provided by Dr. William Jacobs, Albert Einstein University, New York, NY [ 20 ] . The cosmid library contains 30-50 kb fragments of M. tuberculosis genomic DNA generated by partial Sau 3A digestion cloned into Bcl I-digested pYUB178.
The blots were hybridized to pYUB178 plasmid DNA labeled using the ECL Direct Nucleic Acid Labeling and Detection System (Amersham).