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Example sentences for: pyub
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Probing colony blots of the E. coli (pYUB178::H37Rv) library with the 0.8 kb PCR fragment led to the isolation of a 4 kb plasmid.
In the sur2 clone, the amino-terminal 970 bp of the 1128 bp Rv0365c ORF is fused to 14 bp from the pYUB178 vector to generate an ORF encoding 328 amino acids (aa), compared to 376 aa encoded by the full-length Rv0365c ORF.
The pYUB178 plasmid and the λ phage library of pYUB178::H37Rv cosmids were generously provided by Dr. William Jacobs, Albert Einstein University, New York, NY [ 20 ] . The cosmid library contains 30-50 kb fragments of M. tuberculosis genomic DNA generated by partial Sau 3A digestion cloned into Bcl I-digested pYUB178.
The PCR product was used as a probe for colony blots to find cosmids in the E. coli (pYUB178::H37Rv) library carrying at least a portion of the cosmid integrated in the sur2 clone.
In the λ phage library, ~225 cosmids represent one genome-equivalent of M. tuberculosis [ 20 ] . An E. coli (pYUB178::H37Rv) library was created by infecting E. coli XL1-Blue with the λ phage library.
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