Words similar to pyub

Example sentences for: pyub

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The PCR product was used as a probe for colony blots to find cosmids in the E. coli (pYUB178::H37Rv) library carrying at least a portion of the cosmid integrated in the sur2 clone.
About 10 6bacteria of the M. smegmatis (pYUB178::H37Rv) library were inoculated into Middlebrook 7H9 media containing 10 μg kanamycin/mL and then were grown to midlog phase (OD 600 ~ 0.3).
In the sur2 clone, the amino-terminal 970 bp of the 1128 bp Rv0365c ORF is fused to 14 bp from the pYUB178 vector to generate an ORF encoding 328 amino acids (aa), compared to 376 aa encoded by the full-length Rv0365c ORF.
Probing colony blots of the E. coli (pYUB178::H37Rv) library with the 0.8 kb PCR fragment led to the isolation of a 4 kb plasmid.
. The M. smegmatis (pYUB178::H37Rv) library was grown in Middlebrook 7H9 media (Difco) containing 10 μg kanamycin/mL and 0.05% (v/v) Tween 80 (Sigma) or on tryptic soy agar (TSA) (Difco) containing 10 μg kanamycin/mL.