Words similar to pyub
Example sentences for: pyub
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Portions of the E. coli (pYUB178::H37Rv) cosmid library were plated on LB agar containing 50 μg kanamycin/mL, and colony blots were performed with the ECL Direct Nucleic Acid Labeling & Detection System (Amersham) according to manufacturer's instructions.
The PCR product was used as a probe for colony blots to find cosmids in the E. coli (pYUB178::H37Rv) library carrying at least a portion of the cosmid integrated in the sur2 clone.
The E. coli (pYUB178::H37Rv) library was grown in Luria broth (LB) (Difco Laboratories, Detroit, Mich.) containing 50 μg kanamycin/mL (Sigma Chemical Company, St. Louis, Mo.)
The Bam HI/ Eco RI fragment of the M. tuberculosis H37Rv insert of the recovered plasmid was used as a probe in colony blots to find cosmids in the E. coli (pYUB178::H37Rv) library.
In this study, the enrichment process resulted in the isolation of two strains containing small, integrated plasmids rather than the expected 30-50 kb cosmids [ 20 ] . Small plasmids corresponding to the integrated ones were present in the original E. coli (pYUB178:H37Rv) cosmid library.
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