Words similar to pyruvate
Example sentences for: pyruvate
How can you use “pyruvate” in a sentence? Here are some example sentences to help you improve your vocabulary:
THP-1 (generously provided by Dr P Guyre, Dartmouth Medical School), Jurkat (ATCC), and 293 cell lines were cultured in RPMI complete medium (CM), consisting of RPMI 1640 with 10% heat-inactivated fetal bovine serum, 0.1 ml of sodium pyruvate, 2.0 mmol/l of L-glutamine, 25 mmol/l of 1 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), 0.1 mmol/l of nonessential amino acids, 0.25 μg/ml of amphotericin, 50 μg/ml gentamicin (all from BioWhittaker, Walkersville, MD, USA) and 5 × 10 -5M 2-mercaptoethanol (Sigma, St Louis, MO, USA).
For fatty acid labeling, cells in a 6 cm dish were incubated with 1 ml of DMEM containing 10% dialyzed fetal bovine serum, 5 mM sodium pyruvate, and [9,10- 3H]palmitic acid (1 mCi/ml) or [9,10- 3H]myristate (0.
A few tracer studies in the older literature have indicated derivation of α-ketobutyrate from pyruvate rather than from threonine [ 32, 33], and (R)-citramalate synthase activity has been shown recently in M. jannaschii (MJ1392) [ 34].
Examples include A6 kinases [ 17], a number of lipid kinase family members [ 18], aminoglycoside phosphotransferases [ 19], pyruvate dehydrogenase kinase family members [ 20], DNA-dependent protein kinase [ 21], ATM [ 22], ATR [ 23], BCR [ 24, 25], a transient receptor potential channel [ 26] and actin-fragmin kinases [ 27].
Growth medium: NaCl 118, KCl 2.4, creatine 5.7, glucose 5, sodium pyruvate 5, 100× MEM vitamins 10 ml/L, penicillin 100 U/ml, streptomycin 100 μg/ml, 50× MEM essential amino acids 20 ml/L, 100× MEM non-essential amino acids 10 ml/L, HEPES 20, pH 7.4 (with NaOH).
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