Words similar to pyruvate
Example sentences for: pyruvate
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The fba -, pfkAB -, and tpiA - in silico deletion strains had a limited capability to synthesize glyceraldehyde 3-phosphate, 3-phosphoglycerate, phosphoenolpyruvate, pyruvate, acetyl-CoA, α-ketoglutarate, succinyl-CoA, oxaloacetate, and high-energy phosphate bonds in all phases, and were completely unable to synthesize many of the biosynthetic precursors in phases 46 (Table 1) ( tpi - in silico strain discussed below).
Furthermore, the essential glycolytic gene products ( pgk, gapAC ) were required for the synthesis of oxaloacetate, succinyl-CoA, α-ketoglutarate, pyruvate, phosphoenolpyruvate (PEP), and 3-phosphoglycerate within all conditions, and were unable to synthesize all biosynthetic precursors under anaerobic growth conditions.
Various genes involved in cholesterol metabolism (microsomal transfer protein, MTP; apolipoprotein C3, Apoc3; cholesterol 7α hydroxylase, Cyp7a1), carbohydrate metabolism (pyruvate kinase; facilitative glucose transporter gene, GLUT2), and coagulation (vitamin K dependent coagulation factors VII, IX, X) have been previously found to be induced by PPARs [ 32 ] , but were not transcriptionally regulated in the present experiment.
0 × 10 6cells/ml) were incubated in 24-well Falcon plates (Fisher Scientific, Pittsburgh, PA, USA) in RPMI-1640 media containing 7% fetal bovine serum (Life Technologies, Grand Island, NY, USA), 100 U/ml penicillin, 100 μg/ml streptomycin, 2 mM L-glutamine, 50 mM 2-ME, 1 mM sodium pyruvate, 0.01 mM nonessential amino acids, and 10 mM HEPES.
THP-1 (generously provided by Dr P Guyre, Dartmouth Medical School), Jurkat (ATCC), and 293 cell lines were cultured in RPMI complete medium (CM), consisting of RPMI 1640 with 10% heat-inactivated fetal bovine serum, 0.1 ml of sodium pyruvate, 2.0 mmol/l of L-glutamine, 25 mmol/l of 1 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), 0.1 mmol/l of nonessential amino acids, 0.25 μg/ml of amphotericin, 50 μg/ml gentamicin (all from BioWhittaker, Walkersville, MD, USA) and 5 × 10 -5M 2-mercaptoethanol (Sigma, St Louis, MO, USA).
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