Words similar to pyruvate
Example sentences for: pyruvate
How can you use “pyruvate” in a sentence? Here are some example sentences to help you improve your vocabulary:
It appears likely that α-ketobutyrate is generated instead by the 'pyruvate' pathway in which citramalate is generated as the initial intermediate.
Metabolic labeling of cells and immunoprecipitation was performed essentially as described previously [ 21 30 ] . In brief, confluent monolayers of A549 cells were transfected with appropriate dsRNA (at 20 nM final) as described above, and at 8 hr post-transfection, were washed with a phosphate-free buffer (120 mM NaCl, 5 mM KCl, 1.5 mM MgCl 2 , 0.25 mM CaCl 2 , 25 mM NaHCO 3 , 20 mM HEPES [pH 7.4]) and labeled with 32P-orthophosphate (500 μCi/ml) (Amersham) in Dulbecco-modified Eagle MEM lacking both phosphate and pyruvate.
U87 glioma cells were maintained in MEM-Earle's medium with non-essential amino acids, 1 mM sodium pyruvate, 10% FCS, 2 mM Glutamax and gentamycin sulphate in a 10% CO2 environment.
Since the interactions of Ca +2and Mg +2with proteins are difficult to study due to the lack of spectroscopic properties, vanadyl (V IV=O) 2+has been used as a probe of the ligands that compose Mg 2+, Ca 2+, and Mn 2+binding sites of several proteins, including carboxypeptidase [ 16 ] , S-adenosylmethionine synthetase [ 17 18 ] , pyruvate kinase [ 19 20 ] , and F 1 -ATPase [ 21 22 ] . This cation specifically binds to divalent cation binding sites of several enzymes, and in many cases serves as a functional cofactor [ 23 ] . Vanadyl has one axial and four equatorial coordination sites relative to the axis of the double-bounded oxygen, an arrangement that is similar to that for Ca 2+and Mg 2+.
Mutations found in at least two nuclear genes also correlate with Leigh syndrome: one for a subunit of the pyruvate dehydrogenase complex and one for surfeit locus protein 1 (SURF-1), which is involved in the assembly of cytochrome c oxidase.
Loading...