Words similar to pyruvate
Example sentences for: pyruvate
How can you use “pyruvate” in a sentence? Here are some example sentences to help you improve your vocabulary:
THP-1 (generously provided by Dr P Guyre, Dartmouth Medical School), Jurkat (ATCC), and 293 cell lines were cultured in RPMI complete medium (CM), consisting of RPMI 1640 with 10% heat-inactivated fetal bovine serum, 0.1 ml of sodium pyruvate, 2.0 mmol/l of L-glutamine, 25 mmol/l of 1 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), 0.1 mmol/l of nonessential amino acids, 0.25 μg/ml of amphotericin, 50 μg/ml gentamicin (all from BioWhittaker, Walkersville, MD, USA) and 5 × 10 -5M 2-mercaptoethanol (Sigma, St Louis, MO, USA).
Pyruvate conversion into lactate was measured in 0.2 M Tris-HCl (pH 7.2) buffer in the presence of sodium pyruvate (0.
It appears likely that α-ketobutyrate is generated instead by the 'pyruvate' pathway in which citramalate is generated as the initial intermediate.
Growth medium: NaCl 118, KCl 2.4, creatine 5.7, glucose 5, sodium pyruvate 5, 100× MEM vitamins 10 ml/L, penicillin 100 U/ml, streptomycin 100 μg/ml, 50× MEM essential amino acids 20 ml/L, 100× MEM non-essential amino acids 10 ml/L, HEPES 20, pH 7.4 (with NaOH).
These same conclusions regarding energy expenditure were reached in experiments assessing enzymatic activities, and transcriptional changes in rat liver using Northern blots, where fish oil feeding: increased palmitoyl CoA mitochondrial and peroxisomal oxidation rate; increased Cpt1, Cpt2, Δ 3, Δ 2-enoyl CoA isomerase, and Ech1 expression and activity; and decreased expression and activity of FA synthase, malic enzyme, glucose 6-phosphate dehydrogenase, and pyruvate kinase, compared to palm and/or safflower oil feeding [ 93 ] . Likewise, in rat white retroperitoneal adipose tissue, fish oil feeding also decreased Fasn expression [ 94 ] . Intriguingly, the hormone leptin, which also acts through SREBP-1 and PPARα, affected many transcripts similarly to LC-PUFA ( e.g. , leptin decreased Gpam and Apoa4 transcripts; and up regulated Ech1, mitochondrial Hmgcs2, and Cyp4a14 transcripts [ 95 ] .
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