Words similar to pyrophosphate
Example sentences for: pyrophosphate
How can you use “pyrophosphate” in a sentence? Here are some example sentences to help you improve your vocabulary:
Dihydropteroate synthase (DHPS) catalyzes the condensation of p -aminobenzoic acid with 7,8-dihydro-6-hydroxymethylpterin pyrophosphate to give 7,8-dihydropteroate, an intermediate in folate metabolism.
The metal cations direct a 5' nucleoside triphosphate to form a phosphoester bond with the 3' hydroxyl of the preceding nucleotide (the 3'-terminal nucleotide of a growing polynucleotide chain), with the elimination of pyrophosphate [ 6 ] .
Control and treated cells were rinsed twice with PBS and then incubated with 1 ml of lysis buffer (10 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1.5 mM MgCl 2 , 50 mM NaF, 5 mM sodium pyrophosphate, 0.2 mM sodium orthovanadate, 2 μg/ml aprotinin, 2 μg/ml leupeptin, 10% glycerol, 1% Triton X-100, 0.5% NP-40, 1 mM phenylmethylsulfonyl fluoride) for 1 hour with shaking at 4°C.
Cells were scraped into ice-cold lysis buffer: 50 mM HEPES, pH 7.4, 150 mM sodium chloride, 1.5 mM magnesium chloride, 10% glycerol, 1% Triton X-100, 1 mM EGTA, 200 μM sodium vanadate, 10 mM tetrasodium pyrophosphate, 100 mM sodium fluoride, 10 μg/ml leupeptin, and 10 μg/ml aprotinin.
Improvements in sequencing automation and analysis such as capillary electrophoresis have speeded up the process considerably and recent developments such as a sequencing method based on real-time pyrophosphate [ 16 ] , sequencing on microchips [ 17 ] , and massive parallel signature sequencing on microbead arrays [ 18 ] also contribute to the speed and depth of EST gene expression analysis.
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