Words similar to pseudokinase

Example sentences for: pseudokinase

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Within the amino terminal sequences, yeast and other GCN2 proteins have a degenerate kinase domain of unknown function [ 31 ] . There is a 63 amino acid long sequence in IfkA that has 38% identity to the V and VI subdomains of the IfkA/B kinase domain, indicating the presence of an amino terminal pseudokinase domain in the Dictyostelium protein (fig.
In contrast, IfkB begins at the kinase domain and is the only known GCN2-like protein that does not possess a pseudokinase domain.
This region has limited sequence identity to the kinase domain, and thus has been termed the pseudokinase domain [ 37 ] . Similarly, the extension in IfkA shows limited sequence identity to the kinase domain.
A plasmid was constructed that carried 825 bp from the pseudokinase domain of ifkA and 731 bp from the kinase domain, placed at either end of a 1.4 kb blasticidin resistance cassette [ 48 ] . A 3.95 kb EcoR I/ Hind III fragment, containing the ifkA sequences and the blasticidin gene, was released from the plasmid and it's ends were made blunt using the klenow fragment and dephosphorylated to prevent recircularization and reduce nonspecific integration of the fragment.
Attempts to disrupt ifkA were carried out using a plasmid that contained a blasticidin resistance cassette flanked by two fragments of the ifkA gene, each about 800 bp long and derived from the pseudokinase and kinase domains.