Words similar to pseudokinase
Example sentences for: pseudokinase
How can you use “pseudokinase” in a sentence? Here are some example sentences to help you improve your vocabulary:
A plasmid was constructed that carried 825 bp from the pseudokinase domain of ifkA and 731 bp from the kinase domain, placed at either end of a 1.4 kb blasticidin resistance cassette [ 48 ] . A 3.95 kb EcoR I/ Hind III fragment, containing the ifkA sequences and the blasticidin gene, was released from the plasmid and it's ends were made blunt using the klenow fragment and dephosphorylated to prevent recircularization and reduce nonspecific integration of the fragment.
This region has limited sequence identity to the kinase domain, and thus has been termed the pseudokinase domain [ 37 ] . Similarly, the extension in IfkA shows limited sequence identity to the kinase domain.
In contrast, IfkB begins at the kinase domain and is the only known GCN2-like protein that does not possess a pseudokinase domain.
Attempts to disrupt ifkA were carried out using a plasmid that contained a blasticidin resistance cassette flanked by two fragments of the ifkA gene, each about 800 bp long and derived from the pseudokinase and kinase domains.
IfkB is unique among GCN2-like proteins in that it does not possess the amino terminal pseudokinase domain.
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