Example sentences for: protofilament

How can you use “protofilament” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • We have tested 16 site-directed mutants of E. coli FtsZ for assembly and GTPase activity in vitro, and for whether they can function in vivo to complement ftsZ84 . Mutations on the front and back of the FtsZ protofilament were mostly benign: they had normal in vitro assembly, and could complement ftsZ84 . Several of these, however, had significantly reduced in vitro GTPase, demonstrating that the high level of GTPase is not essential for assembly or function in vivo.

  • We observed a second form of protofilament sheet when assembled on cationic lipid monolayers, in which the protofilaments were only 3.7 nm apart [ 2].

  • The straight conformation was seen in single protofilaments assembled from purified FtsZ with GTP or the slowly hydrolyzable analog GMPCPP, and in protofilament sheets assembled with DEAE dextran.

  • Since the FtsZ protofilament appears to be a homolog of the tubulin protofilament [ 2, 11] we will use these same designations for FtsZ.

  • We have not yet examined FtsZ84 for assembly of protofilaments in GTP and protofilament sheets in calcium.


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