Example sentences for: protofilament

How can you use “protofilament” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • We have tested 16 site-directed mutants of E. coli FtsZ for assembly and GTPase activity in vitro, and for whether they can function in vivo to complement ftsZ84 . Mutations on the front and back of the FtsZ protofilament were mostly benign: they had normal in vitro assembly, and could complement ftsZ84 . Several of these, however, had significantly reduced in vitro GTPase, demonstrating that the high level of GTPase is not essential for assembly or function in vivo.

  • The straight conformation was seen in single protofilaments assembled from purified FtsZ with GTP or the slowly hydrolyzable analog GMPCPP, and in protofilament sheets assembled with DEAE dextran.

  • In the presence of GTP, D86K formed a mixture of tubes and straight twin protofilament pairs (Fig.

  • The top and bottom faces of the subunit, which we call longitudinal, contact subunits above and below it in the protofilament; the right and left faces, which we call lateral, contact subunits in adjacent protofilaments; and the front and back faces correspond to the outside and inside of the microtubule.

  • D86K could also form protofilament sheets when assembled in DEAE dextran, as could all of the other lateral mutants (Table 1).


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