Words similar to protofilament

Example sentences for: protofilament

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In GTP plus 10 mM EDTA, or in 10 mM GTP (which also chelates the Mg), the D209A protein assembled into protofilament bundles that formed long spirals or closed circular hoops (Fig.
In the presence of GTP, D86K formed a mixture of tubes and straight twin protofilament pairs (Fig.
These structures seem to be closely related to each other, with the hoops being formed when the protofilament ends join after one turn.
We have tested 16 site-directed mutants of E. coli FtsZ for assembly and GTPase activity in vitro, and for whether they can function in vivo to complement ftsZ84 . Mutations on the front and back of the FtsZ protofilament were mostly benign: they had normal in vitro assembly, and could complement ftsZ84 . Several of these, however, had significantly reduced in vitro GTPase, demonstrating that the high level of GTPase is not essential for assembly or function in vivo.
The straight conformation was seen in single protofilaments assembled from purified FtsZ with GTP or the slowly hydrolyzable analog GMPCPP, and in protofilament sheets assembled with DEAE dextran.