Words similar to protofilament
Example sentences for: protofilament
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We have tested 16 site-directed mutants of E. coli FtsZ for assembly and GTPase activity in vitro, and for whether they can function in vivo to complement ftsZ84 . Mutations on the front and back of the FtsZ protofilament were mostly benign: they had normal in vitro assembly, and could complement ftsZ84 . Several of these, however, had significantly reduced in vitro GTPase, demonstrating that the high level of GTPase is not essential for assembly or function in vivo.
The straight conformation was seen in single protofilaments assembled from purified FtsZ with GTP or the slowly hydrolyzable analog GMPCPP, and in protofilament sheets assembled with DEAE dextran.
In the presence of GTP, D86K formed a mixture of tubes and straight twin protofilament pairs (Fig.
The top and bottom faces of the subunit, which we call longitudinal, contact subunits above and below it in the protofilament; the right and left faces, which we call lateral, contact subunits in adjacent protofilaments; and the front and back faces correspond to the outside and inside of the microtubule.
D86K could also form protofilament sheets when assembled in DEAE dextran, as could all of the other lateral mutants (Table 1).