Words similar to protegrin
Example sentences for: protegrin
How can you use “protegrin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Resistance to polymyxin B is also imparted by the absence or masking of critical binding sites required for polycation-mediated permeabilization of the outer membrane [ 54 ] . Thus, attachment of polymyxin B to Yersinia enterocolitica is reduced by steric hindrance generated by the size and/or branched shape of the outer core region of its LPS macromolecule [ 55 ] . Additionally, resistance to cationic peptides can be due to cytoplasmic clearance of the antimicrobial peptide by an efflux pump; e.g., Yersinia resistance to polymyxin B caused by the RosA/RosB efflux pump/potassium antiporter [ 56 ] and Neisseria gonorrhoeae resistance to protegrin PG-1 via an energy-dependent efflux system termed mtr [ 57 ] .
The indices of susceptibility (C L and MAC values) obtained from our radial diffusion assays established that P. aeruginosa was considerably more sensitive to PG-1 than B. cepacia . We also found that at any given peptide concentration, many fewer protegrin molecules bound to intact B. cepacia and its lipid A than to the corresponding P. aeruginosa preparations.
5show that B. cepacia and P. aeruginosa LPS bound equivalent amounts of PG-1 at high (10-20 μM) protegrin concentrations.
Incubation of 125I-GGPG with intact P. aeruginosa 9027 (PG-1 sensitive) and B. cepacia 25416 (PG-1 resistant) cells demonstrated a marked difference in protegrin binding between the two strains (Fig.
By mixing a constant amount of protegrin (within this linear range) with graded amounts of purified LPS and lipid A, we could readily determine how much of these ligands were needed to reduce the activity of 50 ng of PG-1 by 50% (the EC 50 values).
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