Words similar to propagated

Example sentences for: propagated

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The pTX-MKC2 plasmid was therefore transfected into 3xALA myosin II cells, which are resistant to myosin filament hyperphosphorylation and disassembly due to elimination of phosphorylation target sites in the myosin tail [ 24 ] . The resultant 3xALA/pTX-MKC2 cells could be propagated in suspension culture even after selection for elevated expression in 40 μg/ml G418.
One clone expressing 1925 ± 425 fmoles/mg protein, as assessed by [ 3H]-DPDPE binding, was propagated for use in this study.
Sub-confluent (80-90%) LNCaP cells propagated in T25 flasks were washed with 5 ml of plain RPMI 1640 medium and trypsinized with 5 ml trypsin/EDTA solution (GIBCO-BRL, Gaithersburg, MD).
For purification of FLAG-MHCK-C, 8-10 liters of 3xALA/pTX-MKC2 cells were propagated in suspension culture in HL-5 medium to approximately 5 × 10 6cells/ml.
Cells were propagated in T75 flasks in RPMI-1640 medium supplemented with 25 mM HEPES, 10% fetal calf serum, 200 mM L-glutamine and 40 μg/ml Tobramycin.