Example sentences for: promega

How can you use “promega” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Two units of AMV Reverse Transcriptase (Promega, Madison, WI, USA) and 50 U of M-MLV Reverse Transcriptase (Life Technologies) was added, and the samples were incubated at 37°C for 15 min before heat inactivation at 65°C for 10 min.

  • Luciferase activity was measured in cell lysates using the Dual Luciferase Kit (Promega) following the manufacturer's instructions.

  • The pSV-β-galactosidase control vector (Promega) (2.

  • Following isolation of pure plaques, the insert DNAs were prepared by PCR methods with the following conditions: 100 μl reactions containing 1X Pfu buffer (20 mM Tris-Cl, pH 8.75, 10 mM KCl, 2 mM MgSO 4 , 10 mM (NH 4 ) 2 SO 4 , 0.1% v/v Triton X-100, 100 μg/ml bovine serum albumin), 1 μM λgt11 forward and reverse primers (Promega, Madison, WI), 0.2 mM deoxyribonucleotides, and 5 Units of recombinant Pfu DNA Polymerase (Stratagene, La Jolla, CA).

  • Mutagenesis was carried out using the Altered Sites Mutagenesis System (Promega, Wl) as previously described by us [ 33].


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