Words similar to promega

Example sentences for: promega

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Wizard Plus Minipreps DNA Purification System (Promega, Madison, WI) was used to isolate plasmid DNA from E. coli bacteria and from M. smegmatis bacteria as previously described [ 11 ] . Phosphatase reactions using Calf Intestinal Alkaline Phosphatase (Invitrogen) and ligation reactions using T4 DNA Ligase (Invitrogen) were carried out according to manufacturer's directions.
At first, we made degenerate deoxyoligonuclotide primers corresponding to the conserved peptide sequences GDXHGQ and GDXVDRG of PPs [ 17 ] . An approximately 120 bp PCR product, obtained by using these primers with the Pf 3D7 genomic DNA as template, was cloned in the pGEM-T vector (Promega) using the T/A cloning strategy.
Cells were cultured for 24 hours with CDDO as a pretreatment, or CDDO was added at the same time as 10 ng/ml recombinant IL-1β at time 0 (Promega, Madison, WI, USA).
Wild type 5-HT 3AS mouse receptor cDNA was derived from N1E-115 neuroblastoma cells as previously described [ 24 ] . Mutant receptors were constructed using polymerase chain reaction (Quick change mutagenesis kit, Promega).
All plasmids were prepared with the Wizard midiprep kit (Promega).