Words similar to promega
Example sentences for: promega
How can you use “promega” in a sentence? Here are some example sentences to help you improve your vocabulary:
We digested previously isolated lambda genomic clones of the human FGF8 region [ 22 ] with restriction endonucleases (Promega, Madison, WI) and analyzed them by Southern blotting using a mouse Npm3 cDNA [ 21 ] probe, as previously described [ 20 ] . An 8.4-kb Xho I fragment from these clones that hybridized to the probe was subcloned into pBluescript (Stratagene) for analysis.
Confirmation that resisitant clones expressed IDO antisense RNA was obtained by isolating total RNA from G418 resistant clones, treating with ribonuclease free DNase RQ1 (Promega) and reverse transcribing RNA into cDNA in the presence of an IDO sense primer [ 17].
First-strand cDNAs were prepared from 6 μg of total RNA using a mixture of oligo(dT) 12-18 and random hexamer primers with Superscript reverse transcriptase (Promega, Madison, WI).
RT-PCR was performed using the Promega Access RT-PCR System, following Promega's instructions.
These cells where in turn co-transfected with pSV40/Zeo2 (Invitrogen) and pG5-luc (Promega), encoding firefly luciferase or pGAL4-SEAP and MBT-2 cells were selected in 400 μg/ml Zeocin and Panc02 cells in 500 μg/ml Zeocin.
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