Words similar to promega

Example sentences for: promega

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Antisera against PLCγ2, ERK1, ERK2 and the hormone-binding domain of the estrogen receptor (ER) were from Santa Cruz Biotechnology (Santa Cruz, CA), and anti-active JNK antiserum was from Promega (Madison, WI).
Cell clones were transiently transfected using Superfect reagent with 1 μg per well of 6 well plate with either pFR-luc (Promega) or pGAL4-SEAP (secreted alkaline phosphatase) (provided by Dr. R. Herzig, Rheogene Inc.) and 3 μg carrier DNA (pLITMUS, New England Biolabs).
In situ detection of DNA fragmentation was performed by incorporation of fluorescein-12-dUTP at the 3'-OH ends of DNA using Terminal deoxynucleotidyl Transferase (TUNEL assay) (Apoptosis Detection System, Fluorescein, Promega, Madison, WI), as performed previously by this laboratory [ 7 26 ] . Nuclei were counterstained using propidium iodide (Sigma Chemical Co., St. Louis, MO).
Membranes were then incubated with primary antibodies to various MAPK family members overnight at 4°C (phospho-ERK, 1:5000, Promega; Madison, WI; phospho-p38, 1:1000, New England Biolabs, Beverly, MA; phospho-JNK, 1:500, Santa Cruz Biotechnology, Inc., Santa Cruz, CA).
[ 11 ] . Antisense and sense RNA probes generated from PCR of a 320-bp fragment (nt 1524-1843) from the last exon including the 3' UTR of human SPAM1 were labeled with digoxigenin-11-UTP (DIG) using an in vitro transcription system [Riboprobe ®System, (Promega, Cat.