Words similar to promega

Example sentences for: promega

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Membranes were then incubated with primary antibodies to various MAPK family members overnight at 4°C (phospho-ERK, 1:5000, Promega; Madison, WI; phospho-p38, 1:1000, New England Biolabs, Beverly, MA; phospho-JNK, 1:500, Santa Cruz Biotechnology, Inc., Santa Cruz, CA).
We digested previously isolated lambda genomic clones of the human FGF8 region [ 22 ] with restriction endonucleases (Promega, Madison, WI) and analyzed them by Southern blotting using a mouse Npm3 cDNA [ 21 ] probe, as previously described [ 20 ] . An 8.4-kb Xho I fragment from these clones that hybridized to the probe was subcloned into pBluescript (Stratagene) for analysis.
The vector pRL-TK (Promega), expressing the Renilla luciferase enzyme from the HSV thymidine kinase (TK) promoter, was included as an uninduced constitutively expressed internal control.
The Taq I fragments of HL60 cDNA were ligated with the CT adapter at 16°C overnight with 3 μl (9 units) of T4 ligase (Promega, Madison, WI) while the Taq I fragments of HL60/TLK286 cDNA were ligated with the TT adapter.
Non-radioactive RNA probes were transcribed from multi-probe template set for rat cytokines (rCK-3) and a set for rat apoptosis factors (rAPO-1), (Pharmingen, San Diego, CA) [ 6 ] . An in vitro RNA transcription kit (Promega, Madison, WS) was applied with the addition of biotin-16-UTP for labeling the probes (Roche, Indianapolis, IN).