Words similar to promega
Example sentences for: promega
How can you use “promega” in a sentence? Here are some example sentences to help you improve your vocabulary:
The in vitro transcription was performed for 2 hours at 37°C in reactions containing 100 ng of template DNA, 40 U of T7 RNA polymerase (for antisense probes) or 40 U of SP6 RNA polymerase (for sense probes), 40 mM Tris-HCl (pH 8.0), 6 mM MgCl 2 , 10 mM dithioerythritol, 2 mM spermidine, 10 mM NaCl, 1 mM each of ATP, GTP, and CTP, 0.65 mM UTP, 0.35 mM digoxigenin (DIG)-labeled UTP (or fluorescein-12-UTP), and 20 U RNasin (Promega, Madison, WI).
PCR amplification was performed on 1 μl of RT product (25 μM) incubated with 0.5 U of Taq DNA polymease (Promega) in a 25 μl reaction mixture containing 0.5 μM 10 mM dNTP, 1.5 μl of 25 mM MgCl, 2.5 μl of 10× Taq DNA polymerase buffer from Promega, 10 pmol of internal standard gene upstream and downstream primers to minimize variations in amplification efficiency between tubes.
Total TGF-β 1 was measured after acidification to activate latent TGF-β, followed by neutralization using an ELISA kit from Promega (Madison, WI, USA).
1 mUnits, Roche Molecular Biochemicals, IN) or DNA-PK (20 Units, Promega Corp, Wl) for 5 h at 30°C in the following buffers (40 μl final volume).
Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.
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