Words similar to prodelhb-egf
Example sentences for: prodelhb-egf
How can you use “prodelhb-egf” in a sentence? Here are some example sentences to help you improve your vocabulary:
Construct pGBT9/prodelHB-EGF was unable to interact with pGAD424/Fib-1C EGF#5-COOH (data not shown).
To test whether the fibulin-1C interaction with proHB-EGF maps to the heparin-binding region, a construct, pGBT9/prodelHB-EGF, containing amino acid residues Asp 106-His 159(the entire EGF like region of proHB-EGF and 18 out of 32 residues of the heparin-binding region) [ 2 ] was used in the yeast two-hybrid system (see materials and methods).
Caution must be used with this result however, as the size of the cloned prodelHB-EGF region is small, encoding just 54 amino acids; this may have resulted in a Gal4 fusion protein in which the EGF epitopes in prodelHB-EGF are hidden by the Gal4 subunit and thus not recognized by the GAL4 fusion partner protein (GAL4/Fib-lC EGF #5-COOH).
The PCR product was digested with Eco RI and Bam HI and ligated into the Eco RI and Bam HI restriction sites of pGBT9 to produce construct pGBT9/prodelHB-EGF.