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Example sentences for: processivity
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The replicative polymerase of herpes simplex virus (HSV) is comprised of catalytic (Pol) and processivity subunits (UL42) [ 9 11 ] . HSV Pol is responsible, in concert with other viral proteins, for regulating the fidelity of DNA replication by selecting the correct nucleotides for incorporation and by proofreading or editing mispaired nucleotides [ 20 ] . The Pol subunit contains intrinsic 3'-5' exonuclease activity [ 14 15 16 ] and the N-terminal region of the polypeptide encoding this function is highly conserved in other polymerases, containing three segments referred to as Exo motifs [ 3 26 ] .
This limited detection of SL1 is not surprising as both the poor processivity of reverse transcriptase and the positioning of the vector sequence primer near the beginning of the insert result in low representation of the initial 5' nucleotides of a transcript among EST collections.
Processivity is an important facit of the activity of enzymes that function in complex pathways [ 43 ] . Reduced processivity may explain, for example, the repair deficits in Xeroderma pigmentosum (XPA) cells [ 44 ] . Our molego approach provides a basis for exploring the role of segments of the protein in its functions, rather than relying only on data from missense mutations.
This overlap was less than expected given the redundancy of the cDNA library analyzed here, at nearly 6,000 ESTs, and suggest that: first, libraries made by different methods are likely to result in different representation from an mRNA pool (either different genes or other portions of the same genes as a result of different 5' processivity); and second, that
RSV is a member of the Pneumovirus genus in the Paramyxoviridae family, and contains a nonsegmented negative-stranded RNA genome of about 15 kb [ 5 ] . The minimal viral RNA-dependent RNA polymerase (RdRP) consists of two virally encoded proteins, the large protein L and the phosphoprotein P [ 6 7 8 ] , and a third viral protein, M2-1 (22K), contributes RNA antitermination function and optimal processivity [ 9 ] . In addition to these viral proteins, the RdRP also requires cellular actin.
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