Words similar to primed
Example sentences for: primed
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For each sample to be analyzed by microarray, 4 μg amplified RNA were primed with 5 μg random hexamers and 5 μg oligo dT and reverse-transcribed, incorporating aa-dUTP, as described at http://www.microarrays.org.
After 6 weeks epitope mapping was performed as described using the BPN'Y217L 15-mer PepSet and splenocytes from the primed animals (Figure 5).
T cells from RASF primed with anti-CD28 exhibited a decrease in the percentage of IL-2-producing cells (15% versus 31%) and an increase in the percentage of IFN-γ secreting cells (67% versus 41%) as compared with freshly isolated cells.
Total RNA (100 μg) was fluorescence-labeled by oligo-dT-primed reverse transcription to cDNA in the presence of Cy3-dUTP as described by Eisen and Brown [ 31].
keloid fibroblasts (and to a lesser extent normal non-PP fibroblasts) appear primed to more effectively transmit the TGFβ1 signal and thereby to express the myofibroblast phenotype.
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