Example sentences for: prewarmed

How can you use “prewarmed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After an overnight hybridization, the embryos were washed at high stringency in prewarmed 50% formamide, 5X SSC, 1% SDS (wash I) at 70°C for 90 minutes.

  • The cells were harvested in prewarmed PBS at 37°C, and spun at 1,500 g for 5 min.

  • Exactly one milliliter of fresh prewarmed isotonic medium (~300 mOsM) was added to the lower chamber and exactly 175 μl of hypertonic medium (~450 mOsM) was added to the upper chamber at time zero.

  • To evaluate whether the net1(7m) and net1(19m') phosphosite mutants have an impact on FEAR, we analyzed Cdc14 localization in synchronous populations of dbf2-1 , net1(7m) dbf2-1, and net1(19m') dbf2-1 cells following release from an α-factor block in G1 phase at 25°C to α-factor-free medium prewarmed to 37°C (nonpermissive temperature for dbf2-1 ). As shown in Figure 6D, the percentage of cells that completely released Cdc14 during early anaphase (spindle length 0-8 μm) was reduced by ~50% in net1(7m) and ~25% in net1(19m') cells compared with wild-type cells.

  • At the indicated times, blood was mixed 1:1 with 2% dextran (150 kDa; Polysciences) in prewarmed RPMI and left at 37°C for 20 min to sediment the red blood cells preferentially.


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