Words similar to prewarmed
Example sentences for: prewarmed
How can you use “prewarmed” in a sentence? Here are some example sentences to help you improve your vocabulary:
After an overnight hybridization, the embryos were washed at high stringency in prewarmed 50% formamide, 5X SSC, 1% SDS (wash I) at 70°C for 90 minutes.
Exactly one milliliter of fresh prewarmed isotonic medium (~300 mOsM) was added to the lower chamber and exactly 175 μl of hypertonic medium (~450 mOsM) was added to the upper chamber at time zero.
To evaluate whether the net1(7m) and net1(19m') phosphosite mutants have an impact on FEAR, we analyzed Cdc14 localization in synchronous populations of dbf2-1 , net1(7m) dbf2-1, and net1(19m') dbf2-1 cells following release from an α-factor block in G1 phase at 25°C to α-factor-free medium prewarmed to 37°C (nonpermissive temperature for dbf2-1 ). As shown in Figure 6D, the percentage of cells that completely released Cdc14 during early anaphase (spindle length 0-8 μm) was reduced by ~50% in net1(7m) and ~25% in net1(19m') cells compared with wild-type cells.
The cells were harvested in prewarmed PBS at 37°C, and spun at 1,500 g for 5 min.
At the indicated times, blood was mixed 1:1 with 2% dextran (150 kDa; Polysciences) in prewarmed RPMI and left at 37°C for 20 min to sediment the red blood cells preferentially.