Example sentences for: pretreated

How can you use “pretreated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To determine whether TGF-β1 blocked Hyal-1 and Hyal-2-increased TNF killing, the cells were pretreated with TGF-β1 for 2 hr and then exposed to TNF-α in the continued presence of TGF-β1 for 16-24 hr.

  • Growth curve analysis of Infected populations and were pretreated for 5 days in phenol red-free, Dulbecco's modified eagle medium (DMEM)/F-12 medium (Gibco/Life Technologies) supplemented with 10% charcoal/dextran treated serum (Hyclone, Logan, UT, USA), then plated at 2.5 × 10 6cells per 100mm dish in triplicate in the absence or presence of estrogen (10 -8mol/l; 17β-Estradiol; 1,3,5 (10) - Estratriene 3,17β-diol; Sigma, St Louis, MO, USA).

  • Finally, to evaluate the impact of glutathione (GSH), a known inhibitor of TNFα-induced ROS formation and apoptosis, on CLENDO cell survival, cells were pretreated with control media or GSH (10 mM) for 30 min, and then treated with or without TNFα (50 ng/ml) for 48 h.

  • Cultures were then washed twice with 1X DPBS and pretreated with inhibitors DEX or NAC in serum-free DMEM to final concentrations as indicated in the figures.

  • However, DSLET-mediated ERK activation was unaltered in identically pretreated cultures.


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