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Example sentences for: preparations

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Steady state kinetic studies were performed on multiple preparations of wild type L1, and the resulting kinetic data are shown in Tables 3, 4, 5. When using nitrocefin as substrate and 50 mM cacodylate, pH 7.0, as buffer, wild-type L1 exhibited a k cat value of 38 ± 1 s -1and a K m value of 12 ± 1 μM.
The formazan accumulated intracellularly and could be visualized by epifluorescence microscopy in wet-mount preparations, on polycarbonate membrane filter surfaces, or directly in some biofilms.
Preparations were then tested for the presence of LPS with a Quantitative Chromogenic LAL kit (Cambrex, East Rutherford, New Jersey, United States).
In rodents, intravenous injection of a non-targeted vector - viruses or plasmid preparations - induces mainly transfection in the liver and the spleen [ 1 2 ] . As of today, transfection of other large organs by viral vectors has not been accomplished in by way of systemic delivery [ 3 ] . The main inconveniences of intravenous administration of first generation adenoviral vectors are their immunogenicity [ 4 ] and hepatic toxicity [ 5 6 ] . The muscle as a large tissue mass is a candidate for production of recombinant protein, but the transfection is limited to the injection site.
Finally, preparations were extensively dialyzed against phosphate buffer to remove free glucose.